BioLegend is proud to support a variety of reagents for microscopy imaging of cells and tissue samples. Our reagents include antibody conjugates to bright, photostable fluors like Brilliant Violet 421™ and the Alexa Fluor® dyes. We also provide cell tracking dyes and nuclear counterstains. BioLegend will continue to release new microscopy products as we discover innovative technologies.
Brilliant Violet 421™
Brilliant Violet 421™ (BV421™) allows for expanded options in multicolor microscopy. BV421™ is used in the “blue” channel, which is typically occupied by DAPI or Alexa Fluor® 405. However, since the spectrum of BV421™ is quite distinct from that of DAPI, please check the actual filter wavelength specifications to ensure optimal transmission of excitation and emission energy in filter cubes employed in widefield microscopy.  Commercial filters are now available for BV421™ (table below).  BV421™ is also excellent for confocal microscopy employing a 405nm laser.

Vendors and Catalog Numbers for BV421™ Widefield Filter Sets

ChromaCat. No. 49027
OmegaCat. No. XF403
SemrockCat. No. BV421-3824A-000
(Right) Frozen C57BL/6 mouse spleen was fixed, blocked, and stained with anti-mouse CD169 (clone 3D6.112) Alexa Fluor® 594 (red), anti-mouse B220 (clone RA3-6B2) BV510™ (purple), anti-mouse Ly6G (clone 1A8) Alexa Fluor® 647 (green), anti-mouse CD4 (clone GK1.5) Alexa Fluor® 488 (yellow), and anti-mouse CD8a (clone 53-6.7) BV421™ (aqua). The image was taken with a 10x objective.
Alexa Fluor® 488, Alexa Fluor® 594, and Alexa Fluor® 647
Microscope optics, filters and detection modalities (cooled LCD cameras and confocal PMTs) are becoming ever more efficient, making these microscopes ever more sensitive.  This increase in sensitivity enables the use of directly conjugated antibodies, making multicolor microscopy easier to accomplish by reducing the limitations of species specific secondary detection.  Alexa Fluor® 488, Alexa Fluor® 594, and Alexa Fluor® 647 are popular fluors that provide strong signal and are photostable for imaging. We offer over 280 Alexa Fluor® 488, 90 Alexa Fluor® 594, and 400 Alexa Fluor® 647 directly conjugated antibodies, and these numbers will grow as we find more targets.
(Right) C57BL/6 mouse frozen intestine section was fixed, blocked, and then stained with anti-mouse CD324 (clone DECMA-1) Alexa Fluor® 594 (red). Nuclei were counterstained with DAPI (blue). The image was captured by a 10X objective.
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