- M14-23 (See other available formats)
- Regulatory Status
- Other Names
- LPS receptor
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14 in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse CD14-expressing NRK-52E transfected cells.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with Spark YG™ 593 under optimal conditions.
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Spark YG™ 593 has a maximum excitation of 573 nm and a maximum emission of 593 nm.
- Excitation Laser
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats of this clone) include: blocking.1 M14-23 blocks LPS-binding to CD14/MD4/TLR4 complex in transfected cells.
(PubMed link indicates BioLegend citation)
- Tsukamoto H, et al. 2010. Int. Immunol. 22(4):271. (Block)
AB_2894630 (BioLegend Cat. No. 150107)
AB_2894630 (BioLegend Cat. No. 150108)
- GPI-linked membrane glycoprotein, 53 - 55 kD.
Macrophages, dendritic cells, kupffer cells, and hepatocytes.
- LPS receptor and clearance of Gram-negative pathogens.
- Cell Type
- Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.
- Gene ID
- 12475 View all products for this Gene ID
- View information about CD14 on UniProt.org
|Purified anti-mouse CD14||M14-23||FC|
|Ultra-LEAF™ Purified anti-mouse CD14||M14-23||FC, Block|
|PE anti-mouse CD14||M14-23||FC|
|Spark YG™ 593 anti-mouse CD14||M14-23||FC|
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