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Purified Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-PURE, SAV-PU, SAV
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Product Citations
publications
Cat # Size Price Save
405150 1 mg ¥13,860
405151 10 mg ¥80,850
Description

Streptavidin is a 52.8 kD tetrameric protein obtained from Streptomyces avidinii. It binds to biotin with a very high affinity and is one of the strongest interactions in nature with a dissociation constant of 10-14 mol/L. It is used in a wide range of applications including ELISA, flow cytometry, molecular biology, and bionanotechnology.

Product Details
Technical data sheet

Product Details

Formulation
Each vial contains Streptavidin lyophilized in sodium chloride buffer.
Preparation
Reconstitute the content of the vial in distilled water to the desired concentration.
Storage & Handling
For long-term storage, store the lyophilized streptavidin at -20°C or -70°C. After reconstitution, store the solution between 2°C and 8°C for up to 2 weeks, or store in aliquots at -20°C for up to 6 months.
Application

FC - Quality tested
ELISA Capture, ELISA Detection, ICC, IHC-F, IHC-P, IP, WB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Rajagopalan A, et al. 2021. Cell Rep Methods. 1:. PubMed
  2. Urlaub D, et al. 2020. Curr Protoc Immunol. 131:e111. PubMed
  3. Ridge LA, et al. 2021. Cell Rep. 36:109610. PubMed

Antigen Details

Structure
52.8 kD tetrameric protein
Distribution

Streptavidin binds to biotin with high affinity

Ligand/Receptor
Biotin
Antigen References

1. Dundas CM, et al. 2013. Appl. Microbiol. Biotechnol. 97:9343.
2. Zhao X, et al. 2013. J. Anal. Methods Chem. 2013:581093.
3. Kaplan DL, et al. 1999. Biomol. Eng. 16:135.
4. Wilbur DS, et al. 1999. Biomol. Eng. 16:113.
5. Sano T, et al. 1998. J. Chromatogr. B. Biomed. Sci. Appl. 715:85.

Gene ID
NA
UniProt
View information about Streptavidin on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 12/30/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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