- 15F9 (See other available formats)
- Regulatory Status
- Other Names
- Inducible COStimulatory molecule, H4, CD278
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
CD278 is a 47-57 kD protein, also known as inducible costimulatory molecule (ICOS), or H4. This protein is homologous to the CD28/CTLA-4 proteins. It forms homodimers and is expressed on activated T cells and a subset of thymocytes. It is able to costimulate T cells proliferation. In addition, ICOS has been shown to be involved in humoral immune responses (B cell germinal center formation). The ICOS ligand is B7-H2. ICOS stimulation has been shown to potentiate TCR-mediated IL-4 and IL-10 production and has been proposed to play a role in Th2 cell development. It has also been shown to be involved in airway tolerance and the downregulation of pulmonary inflammation.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Syrian Hamster
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 640 under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire™ 640 has a maximum excitation of 566 nm and a maximum emission of 639 nm.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining2 of acetone-fixed frozen sections.
(PubMed link indicates BioLegend citation)
- Dong C, et al. 2001. Nature 409(6816):97.
- Walker LSK, et al. 2003. J. Immunol. 170:91.
AB_2924445 (BioLegend Cat. No. 107713)
AB_2924445 (BioLegend Cat. No. 107714)
- CD28/CTLA-4, 47-57kD
Activated T cells, subset of thymocytes
- Costimulates T cell activation, proliferation, humoral immune response
- Cell Type
- T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
- Rudd CE, et al. 2003. Nat. Rev. Immunol. 3:544.
- McAdam AJ, et al. 2000. J. Immunol. 165:5035.
- Mak TW, et al. 2003. Nat. Immunol. 4:765
- Gene ID
- 54167 View all products for this Gene ID
- View information about CD278 on UniProt.org
|PE anti-mouse CD278 (ICOS)||15F9||FC|
|PE/Cyanine5 anti-mouse CD278 (ICOS)||15F9||FC|
|APC anti-mouse CD278 (ICOS)||15F9||FC|
|PE/Fire™ 640 anti-mouse CD278 (ICOS)||15F9||FC|
|PE/Fire™ 700 anti-mouse CD278 (ICOS)||15F9||FC|
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