PE/Cyanine5 anti-human CD185 (CXCR5) Antibody

Pricing & Availability
J252D4 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and CD185 (CXCR5) (clone J252D4) PE/Cyainine5 (left) or mouse IgG1, κ PE/Cyanine5 isotype control (right).
  • J252D4_PECy5_CD185_CXCR5_Antibody_091621.png
    Human peripheral blood lymphocytes were stained with anti-human CD19 FITC and CD185 (CXCR5) (clone J252D4) PE/Cyainine5 (left) or mouse IgG1, κ PE/Cyanine5 isotype control (right).
Compare all formats See PE/Cyanine5 spectral data
Cat # Size Price Save
356951 25 tests ¥37,620
356952 100 tests ¥92,840

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human CXCR5-transfected cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine5 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
AB_2894488 (BioLegend Cat. No. 356951)
AB_2894488 (BioLegend Cat. No. 356952)

Antigen Details

G-protein coupled receptor, 7 transmembrane regions, 42 kD

Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.

Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Cell Type
B cells, Tfh
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.

Gene ID
643 View all products for this Gene ID
View information about CD185 on

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 09/16/2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • PE anti-human CD185 (CXCR5)

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  • Purified anti-human CD185 (CXCR5)

  • Alexa Fluor® 647 anti-human CD185 (CXCR5)

  • PerCP/Cyanine5.5 anti-human CD185 (CXCR5)

  • Alexa Fluor® 488 anti-human CD185 (CXCR5)

  • FITC anti-human CD185 (CXCR5)

  • Alexa Fluor® 700 anti-human CD185 (CXCR5)

  • Pacific Blue™ anti-human CD185 (CXCR5)

  • Brilliant Violet 421™ anti-human CD185 (CXCR5)

  • PE/Cyanine7 anti-human CD185 (CXCR5)

  • APC/Cyanine7 anti-human CD185 (CXCR5)

  • PE/Dazzle™ 594 anti-human CD185 (CXCR5)

  • Brilliant Violet 605™ anti-human CD185 (CXCR5)

  • Biotin anti-human CD185 (CXCR5)

  • Brilliant Violet 711™ anti-human CD185 (CXCR5)

  • Brilliant Violet 785™ anti-human CD185 (CXCR5)

  • TotalSeq™-A0144 anti-human CD185 (CXCR5)

  • TotalSeq™-C0144 anti-human CD185 (CXCR5)

  • Brilliant Violet 750™ anti-human CD185 (CXCR5)

  • TotalSeq™-B0144 anti-human CD185 (CXCR5)

  • APC/Fire™ 750 anti-human CD185 (CXCR5)

  • Spark NIR™ 685 anti-human CD185 (CXCR5)

  • TotalSeq™-D0144 anti-human CD185 (CXCR5)

  • PE/Cyanine5 anti-human CD185 (CXCR5)

  • PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody

  • APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody


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