PE anti-Nestin Antibody

Pricing & Availability
10C2 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
HUVEC cells were treated with the True-Nuclear™ Transcription Factor Buffer Set, then stained with Nestin (clone 10C2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 10C2_PE_Nestin_Antibody_123115
    HUVEC cells were treated with the True-Nuclear™ Transcription Factor Buffer Set, then stained with Nestin (clone 10C2) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
Cat # Size Price Save
656805 25 tests ¥28,980
656806 100 tests ¥63,630

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Fusion protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Jozef S, et al. 2012. Biomed. Pap. Med. Fac. 156:135. (IHC)
Product Citations
  1. Kuang YL, et al. 2019. Stem Cell Res. 37:101434. PubMed
  2. López-Valero I, et al. 2020. Theranostics. 10:5120. PubMed
  3. Gerace D, et al. 2021. Stem Cell Research. 53:102371. PubMed
AB_2566381 (BioLegend Cat. No. 656805)
AB_2566382 (BioLegend Cat. No. 656806)

Antigen Details

1621 amino acids with predicted molecular weight of 200-220 kD


Required for survival and renewal of neural progenitor cells; plays roles in angiogenesis and tumor formation
Vimentin, alpha-internexin and FHOD3
Cell Type
Neural Stem Cells
Biology Area
Angiogenesis, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
Intermediate Filaments
Antigen References

1. Matsuda Y, et al. 2013. World J. Gastroenterol. 19:42.
2. Matsuda Y, et al. 2012. Med. Mol. Morphol. 45:59.
3. Krupkova O Jr, et al. 2010. Neoplasma. 57:291.
4. Hombach-Klonisch S, et al. 2008. Arch. Immunol. Ther. Exp. (Warsz). 56:165.
5. Pilkington GJ, et al. 2005. Cell Prolif. 38:423.

Gene ID
10763 View all products for this Gene ID
View information about Nestin on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 09/12/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


8999 BioLegend Way, San Diego, CA 92121
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Nestin

  • Alexa Fluor® 594 anti-Nestin

  • Brilliant Violet 421™ anti-Nestin

  • PE anti-Nestin

  • Alexa Fluor® 647 anti-Nestin

  • Direct-Blot™ HRP anti-Nestin

  • Alexa Fluor® 488 anti-Nestin


Login / Register
Remember me
Forgot your password? Reset password?
Create an Account