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PE anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-18H10.1 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
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Product Citations
publications
TC11-18H10.1_PE_041910
PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10.1_PE_041910
    PMA/ionomycin-stimulated (5 hours) Th17 polarized C57BL/6 mouse CD4+ T cells surface stained with CD4 (GK1.5) APC, then intracellularly stained with TC11-18H10.1 PE
  • TC11-18H10_PE_060806
    PMA (20 ng/ml) + ionomycin (1 µg/ml) -stimulated (6 hours + monensin, 2 µM) mouse thymoma cell line EL-4 intracellularly stained with TC11-18H10.1 PE
Compare all formats See PE spectral data
Cat # Size Price Save
506903 25 µg ¥19,580
506904 100 µg ¥49,840
Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts, activation of NF-κB, and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IL-17A antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture3,4 and ELISPOT Capture5: The purified TC11-18H10.1 antibody is useful as the capture antibody in a sandwich ELISA, when used in conjunction with the biotinylated TC11-8H4 antibody (Cat. No. 507002) as the detecting antibody and recombinant mouse IL-17 (Cat. No. 576009) as the standard.
Flow Cytometry2-4,7,8,11,12: The TC11-18H10.1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-17-producing cells within mixed cell populations.
Neutralization6,9: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IL-17 bioactivity in vivo and in vitro (Cat. No. 506906).

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schubert D, et al. 2004. J. Immunol. 172:4503. (ICFC)
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107. (ICFC, ELISA Capture)
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254. (ICFC, ELISA Capture)
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734. (ELISPOT Capture)
  6. Yen D, et al. 2006. J. Clin. Invest. 116:1310. (Neut)
  7. Ehirchiou D, et al. 2007. J. Exp. Med. 204:1519. (ICFC)
  8. Kang SG, et al. 2007. J. Immunol. 179:3724. (ICFC)
  9. Smith E, et al. 2008. J. Immunol. 181:1357. (Neut) PubMed
  10. Neufert C, et al. 2007. Eur. J. Immunol. 37:1809. PubMed
  11. Wang C, et al. 2009. Mucosal Immunol 2:173. (ICFC) PubMed
  12. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (ICFC) PubMed
  13. Kivisäkk P, et al. 2009. Ann. Neurol. 65:457. PubMed
  14. Cooney LA, et al. 2011. J. Immunol. 187:4440. PubMed
  15. Ma Y, et al. 2012. PLoS One. 7:e40763. PubMed
  16. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
  2. Tian J, et al. 2021. Autophagy. 17:2586. PubMed
  3. Zheng J, et al. 2022. Front Pharmacol. 13:866228. PubMed
  4. Yamazaki S, et al. 2022. Mucosal Immunol. :. PubMed
  5. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  6. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  7. Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
  8. Vono M, et al. 2020. Cell Reports. 28(7):1773-1784.e5.. PubMed
  9. Xue F, et al. 2020. Int J Biol Sci. 2.105555556. PubMed
  10. Files M, et al. 2022. NPJ Vaccines. 7:48. PubMed
  11. Jong RM, et al. 2022. J Immunol. 208:407. PubMed
  12. Kang S, et al. 2007. J Immunol. 179:3724. PubMed
  13. Qian Y, et al. 2021. Cell Reports. 36(8):109602. PubMed
  14. Yang R, et al. 2020. Cell Death Dis. 0.877083333. PubMed
  15. Du C, et al. 2016. Nat Commun. 7: 11120. PubMed
  16. Pan Y, et al. 2021. Front Cell Neurosci. 15:664312. PubMed
  17. Yang C, et al. 2018. J Immunol. 200:1316. PubMed
  18. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  19. Kenichi Shimada et al. 2018. Immunity. 49(5):873-885 . PubMed
  20. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  21. Angiari S, et al. 2020. Cell Metab. 31:391. PubMed
  22. Su N, Xiong Y 2016. Sci Rep. 6:22658. PubMed
  23. Ni J, et al. 2022. Mol Med. 28:65. PubMed
  24. Piper CJM, et al. 2020. Cell Reports. 29(7):1878-1892.e7.. PubMed
  25. Lu Y, et al. 2019. Int J Mol Med. 43:2398. PubMed
  26. Zhao Y, et al. 2015. PLoS One. 10: 0134797. PubMed
  27. Aqel SI, et al. 2021. JCI Insight. 6:e142376. PubMed
  28. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  29. Sehnert B, et al. 2022. Front Immunol. 13:921488. PubMed
  30. Ogawa C et al. 2018. Cell reports. 25(1):19-28 . PubMed
  31. Han Y et al. 2019. Cell reports. 27(3):835-846 . PubMed
  32. Kuwahara M, et al. 2014. Nat Commun. 5:3555. PubMed
  33. Nagaoka M, et al. 2014. J Immunol. 193:2812. PubMed
  34. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  35. Wagner V, et al. 2016. Sci Transl Med. 8: 366ra164. PubMed
  36. D Kim, S Lee, H Jun 2016. Immunol Cell Biol. 10.1038/icb.2016.98. PubMed
  37. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  38. Liu YJ, et al. 2020. Theranostics. 10:5225. PubMed
  39. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  40. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  41. Zhao F, et al. 2022. Front Immunol. 13:873720. PubMed
  42. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  43. Pu Y, et al. 2022. Transl Psychiatry. 12:223. PubMed
  44. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  45. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  46. Luo Y et al. 2019. Cell reports. 26(7):1869-1879 . PubMed
  47. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  48. Dar HY, et al. 2018. Sci Rep. 8:2503. PubMed
  49. Kawakami R, et al. 2021. Immunity. 54(5):947-961.e8. PubMed
  50. Ni P, et al. 2014. Mol Immunol. 62:199. PubMed
  51. Niwa H, et al. 2009. Clin Immunol. 132:184. PubMed
  52. Wang J, et al. 2021. Nat Commun. 12:6198. PubMed
  53. Dar HY, et al. 2018. Bone Rep. 8:46. PubMed
  54. Bhattacharya D, et al. 2014. J Biol Chem. 289:16508. PubMed
  55. Mara AB, et al. 2022. NPJ Vaccines. 7:86. PubMed
  56. Yong L, et al. 2022. Nat Commun. 13:4255. PubMed
  57. Zeng Q, et al. 2022. Front Immunol. 13:740805. PubMed
  58. Takeshita Y, et al. 2021. Neurol Neuroimmunol Neuroinflamm. 8:. PubMed
  59. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  60. Ouyang W, et al. 2021. Invest Ophthalmol Vis Sci. 62:25:00. PubMed
  61. Tan YY, et al. 2021. Exp Ther Med. 22:1368. PubMed
  62. Wen M, et al. 2022. JCI Insight. Online ahead of print. PubMed
  63. Castro–Dopico T, et al. 2020. Cell Reports. 32(1):107857. PubMed
  64. Kawashima H, et al. 2013. J Immunol. 191:3614. PubMed
  65. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  66. Deerhake ME, et al. 2021. Immunity. 54(3):484-498.e8. PubMed
  67. Vaena S, et al. 2021. Cell Reports. 35(5):109076. PubMed
  68. Alhosaini K, et al. 2021. Brain Sci. :11. PubMed
  69. Lin YW, et al. 2022. Mol Med Rep. 26:. PubMed
  70. Park S, et al. 2011. J Immunol. 187:5813. PubMed
  71. Chatterjee S et al. 2017. Cell metabolism. 27(1):85-100 . PubMed
  72. Chou T, et al. 2016. Nat Commun. 7:11904. PubMed
  73. Takeuchi H, et al. 2013. J Immunol. 191:3725. PubMed
  74. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  75. Van Dis E, et al. 2018. Cell Rep. 23:1435. PubMed
  76. Ramakrishnan SK, et al. 2019. Nat Commun. 10:660. PubMed
  77. Katagiri T, et al. 2019. Mucosal Immunol. 12:p1104. PubMed
  78. Xiao S, et al. 2020. Cell Reports. 32(2):107892. PubMed
  79. Ghorbani S, et al. 2022. Nat Commun. 13:2445. PubMed
  80. Gorman JA, et al. 2019. Front Immunol. 10:44. PubMed
  81. Ouyang S, et al. 2019. J Immunol. 202:1441. PubMed
  82. Sanchez M et al. 2017. Cell host & microbe. 22(4):543-551 . PubMed
  83. Luck H, et al. 2019. Nat Commun. 10:3650. PubMed
  84. Metidji A et al. 2018. Immunity. 49(2):353-362 . PubMed
  85. Derada Troletti C, et al. 2021. Cell Reports. 35(9):109201. PubMed
  86. Wang G, et al. 2021. Cell Host Microbe. 29(5):777-791.e6. PubMed
  87. Wu B, et al. 2021. Immunity. 54(2):308-323.e6. PubMed
  88. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  89. Kang J, Lee J, Chang J 2016. PLoS One. 11: 0157015. PubMed
  90. Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed
  91. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  92. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  93. Ehirchiou D, et al. 2007. J Exp Med . 204:1519. PubMed
  94. Li X, et al. 2016. MBio. 7: 02232-15. PubMed
  95. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  96. Mizutani N, et al. 2012. J Immunol. 188:5694. PubMed
  97. Suhail A, et al. 2019. Cell Rep. 29:3522. PubMed
  98. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  99. Zhang Y, et al. 2021. Commun Biol. 344:4. PubMed
  100. Castro‐Dopico T et al. 2019. Immunity. 50(4):1099-1114 . PubMed
  101. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  102. Jeon Y, et al. 2015. PLoS One. 10: e0139845. PubMed
  103. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  104. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  105. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  106. Huang LJ, et al. 2021. Immunity. 54(8):1728-1744.e7. PubMed
  107. Yu H, et al. 2015. PLoS One. 10: 0143001. PubMed
  108. Charlton J, et al. 2015. PLoS One. 10:119200. PubMed
  109. Tang L, et al. 2022. Front Immunol. 12:770402. PubMed
  110. Spiljar M, et al. 2021. Cell Metab. 33:2231. PubMed
  111. Mansouri S, et al. 2021. J Immunol. 206:2233. PubMed
  112. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  113. Wang N, et al. 2020. Front Immunol. 1.765972222. PubMed
  114. Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
  115. Li C, et al. 2014. J Immunol. 192:1425. PubMed
  116. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  117. Toriyama K, et al. 2020. Commun Biol. 0.398611111. PubMed
  118. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  119. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  120. Ge Y, et al. 2020. Mucosal Immunol. 13:34. PubMed
  121. Bing Wu et al. 2018. Immunity. 49(5):886-898 . PubMed
  122. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
  123. Pahari S, et al. 2020. Autophagy. 1.375694444. PubMed
RRID
AB_315463 (BioLegend Cat. No. 506903)
AB_315464 (BioLegend Cat. No. 506904)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian).
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006. Nat. Rev. Immunol. 6:329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237:123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does the anti-mouse IL-17 antibody (clone TC11-18H10.1) recognize the A or F isoform?
Clone TC11-18H10.1 recognizes IL-17A isoform, but it also recognizes the IL-17A/F heterodimer via IL-17A binding.

Other Formats

View All IL-17A Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,ICFC
FITC anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 488 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 647 anti-mouse IL-17A TC11-18H10.1 ICFC
Alexa Fluor® 700 anti-mouse IL-17A TC11-18H10.1 ICFC
APC anti-mouse IL-17A TC11-18H10.1 ICFC
Pacific Blue™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP/Cyanine5.5 anti-mouse IL-17A TC11-18H10.1 ICFC
PE/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 421™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 605™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 650™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 785™ anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 510™ anti-mouse IL-17A TC11-18H10.1 ICFC
Purified anti-mouse IL-17A (Maxpar® Ready) TC11-18H10.1 ELISA Capture,CyTOF®
PE/Dazzle™ 594 anti-mouse IL-17A TC11-18H10.1 ICFC
APC/Cyanine7 anti-mouse IL-17A TC11-18H10.1 ICFC
Brilliant Violet 711™ anti-mouse IL-17A TC11-18H10.1 ICFC
PerCP anti-mouse IL-17A TC11-18H10.1 ICFC
Ultra-LEAF™ Purified anti-mouse IL-17A TC11-18H10.1 ELISA Capture,CyTOF®,ELISPOT Capture,Neut,ICFC
PE/Cyanine5 anti-mouse IL-17A TC11-18H10.1 ICFC
Go To Top Version: 2    Revision Date: 10/16/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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