PE anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
7 reviews
Product Citations
publications
30-F11_PE_090507
C57BL/6 mouse splenocytes stained with 30-F11 PE
  • 30-F11_PE_090507
    C57BL/6 mouse splenocytes stained with 30-F11 PE
See PE spectral data View Moxi Flow Data
Cat # Size Price Save
103105 50 µg ¥7,000
103106 200 µg ¥14,000
Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,  immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103163 and 103164).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Oliver J, et al. 2009. J Am Soc Nephrol. 2.440972222. PubMed
  2. Liu B, et al. 2013. Development. 140:780. PubMed
  3. Aoki H, et al. 2009. Development. 136:2511. PubMed
  4. Guha S, et al. 2014. Brain Behav Immun. 11:123. PubMed
  5. Olson M, et al. 2014. J Immunol. 193:5420. PubMed
  6. Watson N, et al. 2015. J Immunol. 194:2796. PubMed
  7. Vijay R, et al. 2015. J Exp Med. 212: 1851 - 1868. PubMed
  8. Maimets M, et al. 2016. Stem Cell Reports. 6:150-162. PubMed
  9. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  10. Kretschmer I, et al. 2016. J Biol Chem. 291: 4091 - 4106. PubMed
  11. Li Y, Kaneda T 2016. Sci Rep. 6: 25077. PubMed
  12. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  13. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  14. Weng L, et al. 2016. Sci Rep. 6:38632. PubMed
  15. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  16. Gardenier J, et al. 2017. Nat Commun. 8:14345. PubMed
  17. Douam F, et al. 2017. Mbio. 10.1128/mBio.00819-17. PubMed
  18. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  19. Khare P, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.09.002. PubMed
  20. Zhao N, et al. 2018. J Clin Invest. 26:84. PubMed
  21. Hsu JM, et al. 2018. Nat Commun. 9:1908. PubMed
  22. Wen G, et al. 2018. J Am Heart Assoc. 7:e008187. PubMed
  23. Lehmann ML, et al. 2018. Sci Rep. 8:11240. PubMed
  24. Frieler RA, et al. 2017. Exp Neurol. 298:104. PubMed
  25. Djokić V, et al. 2019. Front Immunol. 9:2891. PubMed
  26. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  27. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  28. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  29. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  30. Sardella C, et al. 2018. J Invest Dermatol. 138:500. PubMed
  31. Xu L, et al. 2019. Haematologica. 10.3324/haematol.2018.207258. PubMed
  32. Fang Y, et al. 2019. Aging (Albany NY). 11:4910. PubMed
  33. Ye Z, et al. 2019. Mediators Inflamm. 2019:8709583. PubMed
  34. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  35. Cieslik KA, et al. 2018. J Gerontol A Biol Sci Med Sci. 73:1167. PubMed
  36. Shmeleva EV, et al. 2019. Front Immunol. 10:1780. PubMed
  37. Ogawa M, et al. 2019. J Biol Methods. 5:e102. PubMed
  38. Wang T et al. 2018. Immunity. 49(3):504-514 . PubMed
  39. Stone S et al. 2018. Glia. 66(7):1331-1345 . PubMed
  40. Wang QA et al. 2018. Cell metabolism. 28(2):282-288 . PubMed
  41. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  42. Miska J et al. 2019. Cell reports. 27(1):226-237 . PubMed
  43. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  44. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  45. Simula L et al. 2018. Cell reports. 25(11):3059-3073 . PubMed
  46. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  47. Meixiong J et al. 2019. Immunity. 50(5):1163-1171 . PubMed
  48. Green DP et al. 2019. Neuron. 101(3):412-420 . PubMed
  49. An YA et al. 2017. eLife. 6 pii: e24071. PubMed
  50. Hepler C et al. 2017. eLife. 6 pii: e27669. PubMed
  51. Qi S et al. 2016. eLife. 5 pii: e14756. PubMed
Publication Library
RRID
AB_312970 (BioLegend Cat. No. 103105)
AB_312971 (BioLegend Cat. No. 103106)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
B cells, Dendritic cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 05/14/2013

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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