PE anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
29F.1A12 (See other available formats)
Other Names
PD-1, Programmed Death-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_29F.1A12_PE_100909
Con-A and IL-2 stimulated (3 days) C57BL/6 splenocytes stained with 29F.1A12 PE and CD3 (145-2C11) APC
  • A_29F.1A12_PE_100909
    Con-A and IL-2 stimulated (3 days) C57BL/6 splenocytes stained with 29F.1A12 PE and CD3 (145-2C11) APC
  • B_29F.1A12_PE_100909
    Con A stimulated (3 days) C57BL/6 splenocytes stained with rat IgG2a isotype PE and CD3e (145-2C11) APC
See PE spectral data
Cat # Size Price Save
135205 50 µg ¥20,000
135206 200 µg ¥58,000
Description

CD279, also known as programmed death-1 (PD-1), is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is expressed on activated splenic T and B cells and thymocytes. It is induced on activated myeloid cells as well. PD-1 is involved in lymphocyte clonal selection and peripheral tolerance through binding its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2). It has been reported that PD-1 and PD-L1 interactions are critical to positive selection and play a role in shaping the T cell repertoire. PD-L1 negative costimulation is essential for prolonged survival of intratesticular islet allografts.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue3 and in vivo blocking of PD-1 binding to its ligands2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  2. Lázár-Molnár E, et al. 2008. Proc. Natl. Acad. Sci. USA 105:2658. (Block)
  3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706. (FC, IHC, Block)
  4. Tobias J, et al. 2020. Front Immunol. 11:895 (FC, ELISA) PubMed
Product Citations
  1. Fortner K, et al. 2017. Journal of Autoimmunity. 10.1016/j.jaut.2017.05.003. PubMed
  2. Schell S, et al. 2017. J Immunol. 10.4049/jimmunol.1700611. PubMed
  3. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  4. Lu Y, et al. 2018. Cancer Cell. 33:1048. PubMed
  5. Beloor J et al. 2018. Cell host & microbe. 23(4):549-556 . PubMed
  6. Wang GZ, et al. 2019. Nat Commun. 10:1125. PubMed
  7. Liu J, et al. 2019. J Exp Med. 216:656. PubMed
  8. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  9. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  10. Mintz MA, et al. 2019. Immunity. 51:310. PubMed
  11. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  12. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  13. Russell SL, et al. 2019. Cell Rep. 29:3564. PubMed
  14. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
  15. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  16. Moufarrij S, et al. 2020. Sci Rep. 10:3470. PubMed
  17. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  18. Spitsin S, et al. 2012. Clin Vaccine Immunol. 1.313888889. PubMed
  19. Soni C, et al. 2014. J Immunol. 193:4400. PubMed
  20. Wong E, et al. 2015. J Immunol. 194:4130. PubMed
  21. Domeier P, et al. 2016. J Exp Med. 213: 715 - 732. PubMed
RRID
AB_1877232 (BioLegend Cat. No. 135205)
AB_1877231 (BioLegend Cat. No. 135206)

Antigen Details

Structure
A 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily.
Distribution

Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.

Function
Involved in lymphocyte clonal selection and peripheral tolerance, prolonged survival of allografts.
Ligand/Receptor
B7-H1 (PD-L1) and B7-DC (PD-L2)
Cell Type
B cells, T cells
Biology Area
Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Nishimura H, et al. 2001. Science 291:319
2. Agata Y, et al. 1996. Int. Immunol. 8:765
3. Liang SC, et al. 2003. Eur. J. Immunol. 33:2706
4. Barber DL, et al. 2006. Nature 439:682
5. Keir ME, et al. 2005. J. Immunol. 175:7372
6. Koehn BH. et al. 2008. J Immunol. 181:5313

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 09/11/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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