PE anti-human Perforin Antibody

Pricing & Availability
B-D48 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1
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Product Citations
Human peripheral blood lymphocytes were surface stained with CD56 APC, followed by intracellular staining with Perforin (clone B-D48) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • B-D48_PE_1_100211
    Human peripheral blood lymphocytes were surface stained with CD56 APC, followed by intracellular staining with Perforin (clone B-D48) PE (top) or mouse IgG1, κ PE isotype control (bottom).
  • B-D48_PE_2_100211
Compare all formats See PE spectral data
Cat # Size Price Save
353303 25 tests ¥36,300
353304 100 tests ¥72,380

Perforin is a 70 kD cytolytic protein that is expressed in the cytoplasmic granules of cytotoxic T lymphocytes (CTLs) and natural killer (NK) cells. Perforin is one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Chiu Y, et al. 2016. Sci Rep. 6:19227. PubMed
  2. Figueroa-Romero C, et al. 2022. Front Immunol. 13:773288. PubMed
  3. Jiang P, et al. 2021. Nat Methods. 18:1181. PubMed
  4. Islas-Vazquez L, et al. 2020. Biology (Basel). 9:00. PubMed
  5. Buggert M, et al. 2014. J Immunol. 192:4685. PubMed
  6. Fan Z, et al. 2020. EMBO Mol Med. 12:e11571. PubMed
  7. Brumeanu TD, et al. 2020. bioRxiv. . PubMed
  8. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  9. Vardam-Kaur T, et al. 2021. Oncotarget. 12:2051. PubMed
  10. Bradley T et al. 2018. Cell. 175(2):387-399 . PubMed
  11. Prado-Garcia H, et al. 2017. Cancer Immunol Immunother. 10.1007/s00262-017-1979-x. PubMed
  12. Collins DR, et al. 2021. Immunity. 54:2372. PubMed
  13. Pahl JHW, et al. 2018. Cancer Immunol Res. 0.609027778. PubMed
  14. Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
  15. Patel T, et al. 2017. Cytotechnology. 10.1007/s10616-017-0106-3. PubMed
  16. Dai B, et al. 2021. Cell Reports Medicine. 2(8):100381. PubMed
AB_10915476 (BioLegend Cat. No. 353303)
AB_2616860 (BioLegend Cat. No. 353304)

Antigen Details

70 kD

CTL, NK (cytoplasmic granules)

Mediates targeted cell lysis.
Cell Type
T cells, NK cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family
Antigen References

1. Lieberman J. 2003. Nat. Rev. Immunol. 3:361.
2. Trapani J, et al. 2002. Nat. Rev. Immunol. 2:735.

Gene ID
5551 View all products for this Gene ID
View information about Perforin on

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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