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Biotin anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_Biotin_010912
ConA-stimulated (3days) C57BL/6 splenocytes were stained with biotinylated CD223 (clone C9B7W) (filled histogram) or rat IgG1 isotype control (open histogram), followed by Sav-PE.
  • C9B7W_Biotin_010912
    ConA-stimulated (3days) C57BL/6 splenocytes were stained with biotinylated CD223 (clone C9B7W) (filled histogram) or rat IgG1 isotype control (open histogram), followed by Sav-PE.
Compare all formats
Cat # Size Price Save
125205 50 µg ¥17,980
125206 500 µg ¥59,630
Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Foy S, et al. 2016. PLoS Pathog. 11: 0150084. PubMed
  2. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  3. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
RRID
AB_2133345 (BioLegend Cat. No. 125205)
AB_2133345 (BioLegend Cat. No. 125206)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD223 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-mouse CD223 (LAG-3) C9B7W FC,IP,ELISA
Biotin anti-mouse CD223 (LAG-3) C9B7W FC
PE anti-mouse CD223 (LAG-3) C9B7W FC
APC anti-mouse CD223 (LAG-3) C9B7W FC
PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3) C9B7W FC
Brilliant Violet 785™ anti-mouse CD223 (LAG-3) C9B7W FC
GoInVivo™ Purified anti-mouse CD223 (LAG-3) C9B7W FC,FA,IP,ELISA
Brilliant Violet 421™ anti-mouse CD223 (LAG-3) C9B7W FC
PE/Dazzle™ 594 anti-mouse CD223 (LAG-3) C9B7W FC
Brilliant Violet 650™ anti-mouse CD223 (LAG-3) C9B7W FC
PE/Cyanine7 anti-mouse CD223 (LAG-3) C9B7W FC
TotalSeq™-A0378 anti-mouse CD223 (LAG-3) C9B7W PG
Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3) C9B7W FC,FA,IP,ELISA
TotalSeq™-C0378 anti-mouse CD223 (LAG-3) C9B7W PG
APC/Fire™ 750 anti-mouse CD223 (LAG-3) C9B7W FC
Alexa Fluor® 647 anti-mouse CD223 (LAG-3) C9B7W FC
TotalSeq™-B0378 anti-mouse CD223 (LAG-3) C9B7W PG
Brilliant Violet 711™ anti-mouse CD223 (LAG-3) C9B7W FC
PE/Fire™ 640 anti-mouse CD223 (LAG-3) C9B7W FC
PerCP/Fire™ 806 anti-mouse CD223 (LAG-3) C9B7W FC
PerCP/Fire™ 780 anti-mouse CD223 (LAG-3) C9B7W FC
Spark Red™ 718 anti-mouse CD223 (LAG-3) (Flexi-Fluor™) C9B7W FC
PE/Cyanine5 anti-mouse CD223 (LAG-3) C9B7W FC
PE/Fire™ 810 anti-mouse CD223 (LAG-3) C9B7W FC
Brilliant Violet 605™ anti-mouse CD223 (LAG-3) C9B7W FC
PE/Fire™ 744 anti-mouse CD223 (LAG-3) C9B7W FC
PE/Fire™ 700 anti-mouse CD223 (LAG-3) C9B7W FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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