Loading...

Biotin anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
C9B7W_Biotin_010912
ConA-stimulated (3days) C57BL/6 splenocytes were stained with biotinylated CD223 (clone C9B7W) (filled histogram) or rat IgG1 isotype control (open histogram), followed by Sav-PE.
  • C9B7W_Biotin_010912
    ConA-stimulated (3days) C57BL/6 splenocytes were stained with biotinylated CD223 (clone C9B7W) (filled histogram) or rat IgG1 isotype control (open histogram), followed by Sav-PE.
Compare all formats
Cat # Size Price Save
125205 50 µg ¥15,940
125206 500 µg ¥53,230
Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Foy S, et al. 2016. PLoS Pathog. 11: 0150084. PubMed
  2. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  3. Trefzer A, et al. 2021. Cell Reports. 34(6):108748. PubMed
RRID
AB_961177 (BioLegend Cat. No. 125205)
AB_2133345 (BioLegend Cat. No. 125206)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD223 (LAG-3)

  • Biotin anti-mouse CD223 (LAG-3)

  • PE anti-mouse CD223 (LAG-3)

  • APC anti-mouse CD223 (LAG-3)

  • PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3)

  • Brilliant Violet 785™ anti-mouse CD223 (LAG-3)

  • GoInVivo™ Purified anti-mouse CD223 (LAG-3)

  • Brilliant Violet 421™ anti-mouse CD223 (LAG-3)

  • PE/Dazzle™ 594 anti-mouse CD223 (LAG-3)

  • Brilliant Violet 650™ anti-mouse CD223 (LAG-3)

  • PE/Cyanine7 anti-mouse CD223 (LAG-3)

  • TotalSeq™-A0378 anti-mouse CD223 (LAG-3)

  • Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3)

  • TotalSeq™-C0378 anti-mouse CD223 (LAG-3)

  • APC/Fire™ 750 anti-mouse CD223 (LAG-3)

  • Alexa Fluor® 647 anti-mouse CD223 (LAG-3)

  • TotalSeq™-B0378 anti-mouse CD223 (LAG-3)

  • Brilliant Violet 711™ anti-mouse CD223 (LAG-3)

  • PE/Fire™ 640 anti-mouse CD223 (LAG-3)
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account