Loading...

PE/Cyanine7 anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
C9B7W_PECy7_CD223_Antibody_081517
Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stained with CD223 (clone C9B7W) PE/Cyanine7 (filled histogram) or rat IgG1, ? PE/Cyanine7 isotype control (open histogram)
  • C9B7W_PECy7_CD223_Antibody_081517
    Con A+IL-2-stimulated C57BL/6 splenocytes (3 days) were stained with CD223 (clone C9B7W) PE/Cyanine7 (filled histogram) or rat IgG1, ? PE/Cyanine7 isotype control (open histogram)
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Save
125225 25 µg ¥23,100
125226 100 µg ¥52,080
Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
Product Citations
  1. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
  2. Cribioli E, et al. 2022. Front Immunol. 13:976628. PubMed
  3. Pein M, et al. 2020. Nat Commun. 11:1494. PubMed
  4. He X, et al. 2021. Adv Sci (Weinh). 8:e2103023. PubMed
  5. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  6. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
  7. He X, et al. 2021. J Immunother Cancer. 9:. PubMed
  8. Molgora M, et al. 2020. Cell. 182:886. PubMed
  9. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
  10. Henkle TR, et al. 2021. Cancer Res. 81:4560. PubMed
  11. Habener A, et al. 2021. J Allergy Clin Immunol. 147:2281. PubMed
RRID
AB_2715763 (BioLegend Cat. No. 125225)
AB_2715764 (BioLegend Cat. No. 125226)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/16/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD223 (LAG-3)

  • Biotin anti-mouse CD223 (LAG-3)

  • PE anti-mouse CD223 (LAG-3)

  • APC anti-mouse CD223 (LAG-3)

  • PerCP/Cyanine5.5 anti-mouse CD223 (LAG-3)

  • Brilliant Violet 785™ anti-mouse CD223 (LAG-3)

  • GoInVivo™ Purified anti-mouse CD223 (LAG-3)

  • Brilliant Violet 421™ anti-mouse CD223 (LAG-3)

  • PE/Dazzle™ 594 anti-mouse CD223 (LAG-3)

  • Brilliant Violet 650™ anti-mouse CD223 (LAG-3)

  • PE/Cyanine7 anti-mouse CD223 (LAG-3)

  • TotalSeq™-A0378 anti-mouse CD223 (LAG-3)

  • Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3)

  • TotalSeq™-C0378 anti-mouse CD223 (LAG-3)

  • APC/Fire™ 750 anti-mouse CD223 (LAG-3)

  • Alexa Fluor® 647 anti-mouse CD223 (LAG-3)

  • TotalSeq™-B0378 anti-mouse CD223 (LAG-3)

  • Brilliant Violet 711™ anti-mouse CD223 (LAG-3)

  • PE/Fire™ 640 anti-mouse CD223 (LAG-3)
ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account