APC/Fire™ 750 anti-human CD3 Antibody

Pricing & Availability
Clone
SK7 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
SK7_APCFire750_CD3_Antibody_1_030816
Human peripheral blood lymphocytes were stained with CD19 PE and CD3 (clone SK7) APC/Fire™ 750 (top) or mouse IgG1, κ APC/Fire™ 750 isotype control (bottom).
  • SK7_APCFire750_CD3_Antibody_1_030816
    Human peripheral blood lymphocytes were stained with CD19 PE and CD3 (clone SK7) APC/Fire™ 750 (top) or mouse IgG1, κ APC/Fire™ 750 isotype control (bottom).
  • SK7_APCFire750_CD3_Antibody_2_030816
Compare all formats See APC/Fire™ 750 spectral data
Cat # Size Price Save
344839 25 tests ¥33,000
344840 100 tests ¥69,740
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex, which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NK T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Additional reported application (for the relevant formats) include: immunohistochemical staining of frozen tissue sections4,5,8, immunofluorescent staining6, and Western blotting3.

Application References

(PubMed link indicates BioLegend citation)
  1. Kan EA, et al. 1983. J. Immunol. 131:536.
  2. Wood GS, et al. 1985. Am. J. Pathol. 120:371.
  3. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  4. Haringman JJ, et al. 2005. Arthritis Res. Ther. 7:R862. (IHC)
  5. Carbone A, et al. 1999. Blood 93:2319. (IHC)
  6. Goval JJ, et al. 2006. J. Histochem. Cytochem. 54:75. (IF)
  7. Rutjens E, et al. 2007. J. Immunol. 178:1702.
  8. Kap Y, et al. 2009. J. Histochem. Cytochem. 57:1159. (IHC)
  9. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations
  1. Hohmann MS, et al. 2021. JCI Insight. 6:. PubMed
  2. Ferguson ID, et al. 2022. Nat Commun. 13:4121. PubMed
  3. Harari A, et al. 2021. Cancers (Basel). 13:. PubMed
  4. Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed
  5. Gupta R, et al. 2022. Front Immunol. 13:886442. PubMed
  6. Seamon K, et al. 2020. Front Immunol. 11:396. PubMed
  7. Soldi R, et al. 2020. PLoS One. 15:e0235705. PubMed
  8. Torres-Ruiz J, et al. 2021. Front Immunol. 12:689966. PubMed
  9. Herrera FG, et al. 2019. Int J Radiat Oncol Biol Phys. 103:320. PubMed
  10. Schupp J, et al. 2021. Int J Mol Sci. 22:00. PubMed
  11. Graciotti M, et al. 2020. Vaccines (Basel). 8:00. PubMed
  12. Warthan MD, et al. 2018. Biol Reprod. 98:309. PubMed
  13. Kasatskaya SA, et al. 2020. Elife. :9. PubMed
RRID
AB_2572091 (BioLegend Cat. No. 344839)
AB_2572114 (BioLegend Cat. No. 344840)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ, (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, during thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
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GMP APC anti-human CD3 SK7 FC
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Spark YG™ 593 anti-human CD3 SK7 FC
GMP PerCP anti-human CD3 SK7 FC
Spark Violet™ 500 anti-human CD3 SK7 FC
Brilliant Violet 650™ anti-human CD3 SK7 FC
Spark PLUS UV™ 395 anti-human CD3 SK7 FC
Go To Top Version: 1    Revision Date: 03/15/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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