APC/Cyanine7 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
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Product Citations
publications
11-26c.2a_APCCy7_092110
C57BL/6 splenocytes stained with 11-26c.2a APC/Cyanine7
  • 11-26c.2a_APCCy7_092110
    C57BL/6 splenocytes stained with 11-26c.2a APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Save
405715 25 µg ¥28,380
405716 100 µg ¥71,060
Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3, and spatial biology (IBEX)10,11.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
  10. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  11. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Turner J, Benet Z, and Grigorova I. 2017. J Immunol. 10.4049/jimmunol.1700267. PubMed
  2. Turner JS et al. 2018. Cell reports. 25(6):1395-1403 . PubMed
  3. Waide ML, et al. 2020. Cell Rep. 33:108503. PubMed
  4. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  5. Yang Y, et al. 2021. Nat Commun. 12:525. PubMed
  6. Proudhon C, et al. 2016. Cell Rep. 15:2159-2169. PubMed
  7. Shan M et al. 2018. Immunity. 49(4):709-724 . PubMed
  8. Geng T, et al. 2022. Methods Mol Biol. 2585:71. PubMed
  9. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  10. Fallet B, et al. 2020. Cell Rep. 30:1013. PubMed
  11. Wilmore JR et al. 2018. Cell host & microbe. 23(3):302-311 . PubMed
  12. Prados A, et al. 2021. Nat Immunol. 22:510. PubMed
  13. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  14. Reynolds A, et al. 2015. J Immunol. 194:231. PubMed
  15. Webster P, et al. 2018. Nat Commun. 9:2649. PubMed
  16. Saumyaa S, et al. 2013. Infect Immun . 81:3426. PubMed
  17. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  18. Alameh MG, et al. 2021. Immunity. 54:2877. PubMed
  19. Nanou A, et al. 2021. Cell Reports. 35(8):109168. PubMed
  20. Xiao G, et al. 2018. Cell. 173:470. PubMed
  21. Scally S, et al. 2017. Nat Commun. . 10.1038/s41467-017-01924-3. PubMed
  22. Mandal RK, et al. 2021. Cell Reports. 35(6):109094. PubMed
  23. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  24. Zhang H, et al. 2021. Cell Reports. 35(6):109096. PubMed
  25. Ghezraoui H, et al. 2018. Nature. 560:122. PubMed
  26. Wilmore JR, et al. 2022. Front Immunol. 12:791095. PubMed
  27. Burnett DL, et al. 2021. Immunity. S1074-7613:00457. PubMed
  28. Lazarian G, et al. 2021. Cancer Cell. 39(3):380-393.e8. PubMed
  29. Sebina I, et al. 2016. PLoS Pathog. 12:e1005999. PubMed
  30. Palmer V, et al. 2015. J Immunol. 195: 1524-1537. PubMed
  31. Schardt JS, et al. 2021. Sci Rep. 11:20738. PubMed
  32. Jones D, et al. 2015. J Immunol. 195: 4753 - 4759. PubMed
  33. Niemeyer BF, et al. 2021. PLoS Pathog. 17:e1010019. PubMed
  34. Hodgson R, et al. 2022. Commun Biol. 5:1216. PubMed
  35. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
RRID
AB_10660304 (BioLegend Cat. No. 405715)
AB_10662544 (BioLegend Cat. No. 405716)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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