- 9E9A8 (See other available formats)
- Regulatory Status
- Other Names
- Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD209, known as Dendritic Cell-Specific Intercellular adhesion molecule 3 (ICAM-3)-Grabbing Nonintegrin (DC-SIGN), is a 44 kD type II transmembrane glycoprotein and a member of the C-type lectin family. CD209 is expressed on myeloid dendritic cells, placental macrophages, liver and placental endothelial cells. CD209 binds to ICAM-3 (CD50), ICAM-2 (CD102), and Butyrophilin (BTN2A1), and mediates dendritic cell migration and T cell proliferation. Importantly, CD209 is a receptor of HIV-1 and some other viruses (such as West Nile virus, hepatitis C virus, etc), and some bacteria or parasites. It plays a critical role in capturing and internalizing those pathogens. LSP1 (leukocyte-specific protein 1) interacts with the cytoplasmic domain of CD209 and mediates transport of HIV to the proteasome.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Extracellular domain of human DC-SIGN
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
ICC - Verified
SB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry on frozen tissue sections1 and spatial biology (IBEX)2,3.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
(PubMed link indicates BioLegend citation)
- Product Citations
AB_1186094 (BioLegend Cat. No. 330111)
AB_1186092 (BioLegend Cat. No. 330112)
- Cell Type
- Dendritic cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Granelli-Piperno A, et al. 2005. J Immunol. 175:4265.
- Gene ID
- 30835 View all products for this Gene ID
- View information about CD209 on UniProt.org
- If an antibody clone has been previously successfully used in IBEX in one fluorescent format, will other antibody formats work as well?
It’s likely that other fluorophore conjugates to the same antibody clone will also be compatible with IBEX using the same sample fixation procedure. Ultimately a directly conjugated antibody’s utility in fluorescent imaging and IBEX may be specific to the sample and microscope being used in the experiment. Some antibody clone conjugates may perform better than others due to performance differences in non-specific binding, fluorophore brightness, and other biochemical properties unique to that conjugate.
- Will antibodies my lab is already using for fluorescent or chromogenic IHC work in IBEX?
Fundamentally, IBEX as a technique that works much in the same way as single antibody panels or single marker IF/IHC. If you’re already successfully using an antibody clone on a sample of interest, it is likely that clone will have utility in IBEX. It is expected some optimization and testing of different antibody fluorophore conjugates will be required to find a suitable format; however, legacy microscopy techniques like chromogenic IHC on fixed or frozen tissue is an excellent place to start looking for useful antibodies.
- Are other fluorophores compatible with IBEX?
Over 18 fluorescent formats have been screened for use in IBEX, however, it is likely that other fluorophores are able to be rapidly bleached in IBEX. If a fluorophore format is already suitable for your imaging platform it can be tested for compatibility in IBEX.
- The same antibody works in one tissue type but not another. What is happening?
Differences in tissue properties may impact both the ability of an antibody to bind its target specifically and impact the ability of a specific fluorophore conjugate to overcome the background fluorescent signal in a given tissue. Secondary stains, as well as testing multiple fluorescent conjugates of the same clone, may help to troubleshoot challenging targets or tissues. Using a reference control tissue may also give confidence in the specificity of your staining.
- How can I be sure the staining I’m seeing in my tissue is real?
In general, best practices for validating an antibody in traditional chromogenic or fluorescent IHC are applicable to IBEX. Please reference the Nature Methods review on antibody based multiplexed imaging for resources on validating antibodies for IBEX.
|Purified anti-human CD209 (DC-SIGN)||9E9A8||FC, IHC-F|
|FITC anti-human CD209 (DC-SIGN)||9E9A8||FC|
|PE anti-human CD209 (DC-SIGN)||9E9A8||FC|
|APC anti-human CD209 (DC-SIGN)||9E9A8||FC|
|PerCP/Cyanine5.5 anti-human CD209 (DC-SIGN)||9E9A8||FC|
|Alexa Fluor® 647 anti-human CD209 (DC-SIGN)||9E9A8||FC, ICC, SB|
|PE/Cyanine7 anti-human CD209 (DC-SIGN)||9E9A8||FC|
|APC/Fire™ 750 anti-human CD209 (DC-SIGN)||9E9A8||FC|
|Brilliant Violet 421™ anti-human CD209 (DC-SIGN)||9E9A8||FC|
|TotalSeq™-A0597 anti-human CD209 (DC-SIGN)||9E9A8||PG|
|TotalSeq™-C0597 anti-human CD209 (DC-SIGN)||9E9A8||PG|
|TotalSeq™-B0597 anti-human CD209 (DC-SIGN)||9E9A8||PG|
|TotalSeq™-D0597 anti-human CD209 (DC-SIGN)||9E9A8||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD209 (DC-SIGN)
FITC anti-human CD209 (DC-SIGN)
PE anti-human CD209 (DC-SIGN)
APC anti-human CD209 (DC-SIGN)
PerCP/Cyanine5.5 anti-human CD209 (DC-SIGN)
Alexa Fluor® 647 anti-human CD209 (DC-SIGN)
PE/Cyanine7 anti-human CD209 (DC-SIGN)
APC/Fire™ 750 anti-human CD209 (DC-SIGN)
Brilliant Violet 421™ anti-human CD209 (DC-SIGN)
TotalSeq™-A0597 anti-human CD209 (DC-SIGN)
TotalSeq™-C0597 anti-human CD209 (DC-SIGN)
TotalSeq™-B0597 anti-human CD209 (DC-SIGN)
TotalSeq™-D0597 anti-human CD209 (DC-SIGN)