PE anti-human CD19 Antibody

Pricing & Availability
Clone
HIB19 (See other available formats)
Workshop
V CD19.11
Other Names
B4
Isotype
Mouse IgG1, κ
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Product Citations
publications
HIB19_PE_061807
Human peripheral blood lymphocytes stained with HIB19 PE
  • HIB19_PE_061807
    Human peripheral blood lymphocytes stained with HIB19 PE
See PE spectral data
Cat # Size Price Save
302207 25 tests ¥5,000
302208 100 tests ¥12,000
302254 100 µg ¥12,000
Description

CD19 is a 95 kD type I transmembrane glycoprotein also known as B4. It is a member of the immunoglobulin superfamily expressed on B-cells (from pro-B to blastoid B cells, absent on plasma cells) and follicular dendritic cells. CD19 is involved in B cell development, activation, and differentiation. CD19 forms a complex with CD21 (CR2) and CD81 (TAPA-1), and functions as a BCR co-receptor.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Chimpanzee, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
µg sizes: 0.2 mg/ml
test sizes: lot-specific (please contact technical support for concentration and total µg amount)
Storage & Handling
The CD19 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections8 and blocking of B cell proliferation. Clone HIB19 is not recommended for formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 302267 & 302268).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Bradbury L, et al. 1993. J. Immunol. 151:2915.
  4. Joseph A, et al. 2010. J. Virol. 84:6645. PubMed
  5. Wang X, et al. 2010. Haematologica. 95:884. (FC) PubMed
  6. Walker JD, et al. 2009. J. Immunol. 182:1548. (Block) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Hansen A, et al. 2002. Arthritis Rheum. 46:2160. (IHC)
  9. Stoeckius M, et al. 2017. Nat. Methods. 14:865. (PG)
  10. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Zheng Q, et al. 2017. Mol Oncol. 10.1002/1878-0261.12056. PubMed
  2. Joly P, et al. 2017. PLoS One. 12(6):e0180568. PubMed
  3. Basnett J, et al. 2017. PLoS One. . 10.1371/journal.pone.0188042. PubMed
  4. BÖiers C, et al. 2018. Dev Cell. 44:362. PubMed
  5. Palazzo A, et al. 2018. J Immunol. 200:2304. PubMed
  6. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  7. Xiao X, et al. 2019. Cell Discov. 5:2. PubMed
  8. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  9. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  10. Cho A, et al. 2019. Cell Rep. 28:909. PubMed
  11. Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed
  12. Zumaquero E, et al. 2019. Elife. 8. PubMed
  13. Dangaj D, et al. 2019. Cancer Cell. 35:885. PubMed
  14. Zhang F, et al. 2019. Immunity. 50:738. PubMed
  15. Luong-Gardiol N, et al. 2019. Cancer Cell. 35:649. PubMed
  16. Jiang Q et al. 2019. Cancer cell. 35(1):81-94 . PubMed
  17. Gomes AM, et al. 2018. Cell Rep. 25:2821. PubMed
  18. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  19. Woodberry T, et al. 2017. Infect Immun. 85:e00986. PubMed
  20. Greiner V, et al. 2019. iScience. 0.75625. PubMed
  21. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Li W, et al. 2020. Immunity. 53(2):456-470. PubMed
  24. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  25. Lavaert M, et al. 2020. Immunity. 52(6):1088-1104. PubMed
  26. Huang Y, et al. 2020. Cancer Cell. 36(6):630-644.e9.. PubMed
  27. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  28. Yamanaka M, et al. 2009. Glycobiology. 1.375694444. PubMed
  29. White C, et al. 2014. J Immunol. 193:5933. PubMed
  30. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  31. Matsui K, et al. 2015. PLoS One. 10: 0137195. PubMed
  32. Bigler M, et al. 2015. PLoS One. 10: 0145635. PubMed
  33. McComb S, et al. 2016. Sci Transl Med. 8: 339ra70. PubMed
RRID
AB_314237 (BioLegend Cat. No. 302207)
AB_314238 (BioLegend Cat. No. 302208)
AB_2564142 (BioLegend Cat. No. 302254)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 95 kD
Distribution

B lineage (except plasma cells), follicular dendritic cells

Function
B cell activation and differentiation
Ligand/Receptor
Forms complex with CD21 (CR2) and CD81 (TAPA-1), BCR coreceptor
Cell Type
B cells, Dendritic cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Tedder T, et al. 1994. Immunol. Today 15:437.
2. Bradbury L, et al. 1993. J. Immunol. 151:2915.

Gene ID
930 View all products for this Gene ID
UniProt
View information about CD19 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 12/18/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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