- YN1/1.7.4 (See other available formats)
- Regulatory Status
- Other Names
- ICAM-1, Ly-47
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
CD54 is a 90 kD immunoglobulin superfamily member also known as ICAM-1 and Ly-47. It is expressed on activated endothelial cells, high endothelial venules (HEV), T and B cells, monocytes/ macrophages, granulocytes, and dendritic cells. CD54 is an important intracellular adhesion molecule that participates in T cell-T cell, T cell-B cell, and T cell-target cell interactions via binding of LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). CD54 has also been shown to be involved in lymphocyte trafficking, making it an important molecule in many immune reactions and inflammation. CD54 is also a receptor for rhinovirus. The YN1/1.7.4 antibody has been reported to block binding of mouse CD54 to LFA-1 and Mac-1, inhibit cell-cell adhesion, and function in antigen presentation to T cells and leukocyte migration to inflammatory tissues.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse NS-1 cells
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC - Quality tested
IP, WB - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro and in vivo blocking of cell-cell adhesion and CD54 functions1,2,4, immunohistochemical staining3 of acetone-fixed frozen sections, immunoprecipitation4, and Western blotting (non-reducing). The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 116131-116136).
- Application References
- Kumaska T, et al. 1996. J. Clin. Invest. 97:2362. (Block)
- Horley KJ, et al. 1989. EMBO J. 8:2889. (Block)
- Burns AR, et al. 1994. J. Immunol. 153:3189. (IHC)
- Takei F, et al. 1985. J. Immunol. 134:1403. (Block, IP)
- Sumagin R, et al. 2010. J. Immunol. 184:5242. PubMed
- Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
- Seidler DG, et al. 2011 J. Immunol. 187:6108. PubMed
- Chacko AM, et al. 2011. Curr Opin Colloid Interface Sci. 16:215. PUbMed
- El-Assaad F, et al. 2013. Infect Immun. 81:3984. PubMed
- Greineder CF, et al. 2013. PLoS One. 14:80110. PubMed
- Product Citations
AB_2813980 (BioLegend Cat. No. 116131)
AB_2813981 (BioLegend Cat. No. 116132)
AB_2813982 (BioLegend Cat. No. 116133)
AB_2813983 (BioLegend Cat. No. 116134)
AB_2813984 (BioLegend Cat. No. 116135)
AB_2813985 (BioLegend Cat. No. 116136)
- Ig superfamily, 90 kD
Activated endothelial cells, high endothelial venules, T cells and B cells, monocytes/macrophages, granulocytes, dendritic cells
- Immune reaction, inflammation, adhesion
- CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Mesenchymal Stem Cells, Monocytes, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1990. Nature 346:425.
3. Rothlein R, et al. 1986. J. Immunol. 137:1270.
- Gene ID
- 15894 View all products for this Gene ID
- View information about CD54 on UniProt.org
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
|Purified anti-mouse CD54||YN1/1.7.4||FC,IHC-F,IP,WB|
|Biotin anti-mouse CD54||YN1/1.7.4||FC|
|FITC anti-mouse CD54||YN1/1.7.4||FC|
|PE anti-mouse CD54||YN1/1.7.4||FC|
|Alexa Fluor® 488 anti-mouse CD54||YN1/1.7.4||FC|
|Alexa Fluor® 647 anti-mouse CD54||YN1/1.7.4||FC|
|Pacific Blue™ anti-mouse CD54||YN1/1.7.4||FC|
|APC anti-mouse CD54||YN1/1.7.4||FC|
|PerCP/Cyanine5.5 anti-mouse CD54||YN1/1.7.4||FC|
|PE/Cyanine7 anti-mouse CD54||YN1/1.7.4||FC|
|APC/Fire™ 750 anti-mouse CD54||YN1/1.7.4||FC|
|TotalSeq™-A0074 anti-mouse CD54||YN1/1.7.4||PG|
|PE/Dazzle™ 594 anti-mouse CD54||YN1/1.7.4||FC|
|Ultra-LEAF™ Purified anti-mouse CD54||YN1/1.7.4||FC,IP,WB|
|TotalSeq™-C0074 anti-mouse CD54||YN1/1.7.4||PG|
|TotalSeq™-B0074 anti-mouse CD54||YN1/1.7.4||PG|
|Brilliant Violet 421™ anti-mouse CD54||YN1/1.7.4||FC|
|Brilliant Violet 711™ anti-mouse CD54||YN1/1.7.4||FC|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse CD54
Biotin anti-mouse CD54
FITC anti-mouse CD54
PE anti-mouse CD54
Alexa Fluor® 488 anti-mouse CD54
Alexa Fluor® 647 anti-mouse CD54
Pacific Blue™ anti-mouse CD54
APC anti-mouse CD54
PerCP/Cyanine5.5 anti-mouse CD54
PE/Cyanine7 anti-mouse CD54
APC/Fire™ 750 anti-mouse CD54
TotalSeq™-A0074 anti-mouse CD54
PE/Dazzle™ 594 anti-mouse CD54
Ultra-LEAF™ Purified anti-mouse CD54
TotalSeq™-C0074 anti-mouse CD54
TotalSeq™-B0074 anti-mouse CD54
Brilliant Violet 421™ anti-mouse CD54
Brilliant Violet 711™ anti-mouse CD54