- G043H7 (See other available formats)
- Regulatory Status
- Other Names
- BLR2, CDw197, EBI1, CMKBR7
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CCR7, also known as CD197, is a chemokine receptor that binds CCL19 and CCL21. CCR7 and its ligands link innate and adaptive immunity by affecting interactions between T cells and dendritic cells and their downstream effect. Naïve T cells enter the lymph node through high endothelial venules, which express CCL21. Dendritic cells and macrophages enter the lymph node through afferent lymphatics. The encounter of T cells and dendritic cells in the T cell zone is CCR7-dependent. In addition, during immunological surveillance, B cells recirculate between B-cell-rich compartments (follicles or B cell zones) in secondary lymphoid organs, surveying for antigen. After antigen binding, B cells move to the boundary of B and T zones to interact with T-helper cells; this B cell migration is directed by CCR7 and its ligands. CCR7-positive cancer cell expression has been associated with lymph node metastasis.Product Details
- Verified Reactivity
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- CCR7-transfected cells
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
FC, Block - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
AB_2814290 (BioLegend Cat. No. 353255)
AB_2814291 (BioLegend Cat. No. 353256)
- Chemokine receptor, G protein-coupled receptors (GPCR), seven transmembrane receptor.
T cells, B cells, NK, dendritic cells.
- The chemokine receptor CCR7 plays a pivotal role in the homing of naïve T cells and regulatory T cells to secondary lymphoid organs, and the migration of dendritic cells into afferent lymphatic vessels.
- CCL19 and CCL21.
- Cell Type
- B cells, Dendritic cells, NK cells, T cells
- Biology Area
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
1. Yanagihara S, et al. 1998. J. Immunol. 161:3096.
2. Charo IF, et al. 2006. N. Engl. J. Med. 354:610.
3. Reif K, et al. 2002. Nature 416:94.
4. Nakata B, et al. 2008. Oncology 74:69.
5. Brodie T. et al. 2013. Cytometry A. 6: 530-2. PubMed
6. Graves A.J. et al. 2014. Cytometry A. 7: 576–9 PubMed
7. Moncunill G. et al. 2014. Cytometry A. 12: 995-8 PubMed
- Gene ID
- 1236 View all products for this Gene ID
- View information about CD197 on UniProt.org
Related Pages & Pathways
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- Do you guarantee that your antibodies are totally pathogen free?
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other FormatsView All CD197 Reagents Request Custom Conjugation
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD197 (CCR7)
Alexa Fluor® 488 anti-human CD197 (CCR7)
Brilliant Violet 421™ anti-human CD197 (CCR7)
PE anti-human CD197 (CCR7)
APC/Cyanine7 anti-human CD197 (CCR7)
Pacific Blue™ anti-human CD197 (CCR7)
APC anti-human CD197 (CCR7)
FITC anti-human CD197 (CCR7)
Alexa Fluor® 647 anti-human CD197 (CCR7)
PerCP/Cyanine5.5 anti-human CD197 (CCR7)
Brilliant Violet 605™ anti-human CD197 (CCR7)
PE/Cyanine7 anti-human CD197 (CCR7)
Brilliant Violet 711™ anti-human CD197 (CCR7)
Brilliant Violet 785™ anti-human CD197 (CCR7)
Brilliant Violet 510™ anti-human CD197 (CCR7)
Brilliant Violet 650™ anti-human CD197 (CCR7)
PE/Dazzle™ 594 anti-human CD197 (CCR7)
Biotin anti-human CD197 (CCR7)
Purified anti-human CD197 (CCR7) (Maxpar® Ready)
PerCP anti-human CD197 (CCR7)
Alexa Fluor® 700 anti-human CD197 (CCR7)
APC/Fire™ 750 anti-human CD197 (CCR7)
TotalSeq™-A0148 anti-human CD197 (CCR7)
TotalSeq™-B0148 anti-human CD197 (CCR7)
TotalSeq™-C0148 anti-human CD197 (CCR7)
Brilliant Violet 750™ anti-human CD197 (CCR7)
Ultra-LEAF™ Purified anti-human CD197 (CCR7)
Spark NIR™ 685 anti-human CD197 (CCR7)
KIRAVIA Blue 520™ anti-human CD197 (CCR7)
PE/Fire™ 640 anti-human CD197 (CCR7)
Spark YG™ 581 anti-human CD197 (CCR7)
APC/Fire™ 810 anti-human CD197 (CCR7) Antibody
TotalSeq™-D0148 anti-human CD197 (CCR7)
PE/Fire™ 810 anti-human CD197 (CCR7) Antibody
PE/Cyanine5 anti-human CD197 (CCR7)