- RMM-1 (See other available formats)
- Regulatory Status
- Other Names
- Immunoglobulin M
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
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IgM is the first immunoglobulin made by B cells in the immune response. Surface IgM is expressed on the majority of mature B cells.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Mouse Ig cocktail
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
- The antibody was purified by affinity chromatography.
- 1.0 mg/ml
- Storage & Handling
- The IgM antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
CyTOF® - Verified
- Recommended Usage
This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
- Application Notes
The RMM-1 antibody reacts with both soluble and membrane immunoglobulin M in all tested mouse haplotypes (Igh-a and b). It does not react with other isotypes. Some formats of the RMM-1 antibody may be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.
- Additional Product Notes
Maxpar® is a registered trademark of Standard BioTools Inc.
- Application References
- Tertilt C, et al. 2009. Infect. Immun. 77:3044. (ELISA) PubMed
- Product Citations
AB_2563777 (BioLegend Cat. No. 406527)
- Ig family
- B cell differentiation, humoral immune response; cross-linking surface IgM induces apoptosis
- Cell Type
- B cells
- Biology Area
- Gene ID
- 16019 View all products for this Gene ID
- View information about IgM on UniProt.org
Related Pages & Pathways
- Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.
- Can I use Maxpar® Ready format clones for flow cytometry staining?
We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
- I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
- Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.
Other FormatsView All IgM Reagents Request Custom Conjugation
|Purified anti-mouse IgM||RMM-1||FC,ELISA|
|PE anti-mouse IgM||RMM-1||FC|
|Biotin anti-mouse IgM||RMM-1||ELISA,FC|
|FITC anti-mouse IgM||RMM-1||FC|
|APC anti-mouse IgM||RMM-1||FC|
|PerCP/Cyanine5.5 anti-mouse IgM||RMM-1||FC|
|APC/Cyanine7 anti-mouse IgM||RMM-1||FC|
|Brilliant Violet 421™ anti-mouse IgM||RMM-1||FC|
|PE/Cyanine7 anti-mouse IgM||RMM-1||FC|
|Alexa Fluor® 488 anti-mouse IgM||RMM-1||FC|
|Brilliant Violet 605™ anti-mouse IgM||RMM-1||FC|
|Alexa Fluor® 647 anti-mouse IgM||RMM-1||FC|
|Purified anti-mouse IgM (Maxpar® Ready)||RMM-1||FC,CyTOF®|
|PE/Dazzle™ 594 anti-mouse IgM||RMM-1||FC|
|Brilliant Violet 510™ anti-mouse IgM||RMM-1||FC|
|TotalSeq™-A0450 anti-mouse IgM||RMM-1||PG|
|Brilliant Violet 711™ anti-mouse IgM||RMM-1||FC|
|APC/Fire™ 750 anti-mouse IgM||RMM-1||FC|
|TotalSeq™-C0450 anti-mouse IgM||RMM-1||PG|
|PE/Cyanine5 anti-mouse IgM||RMM-1||FC|
|TotalSeq™-B0450 anti-mouse IgM||RMM-1||PG|
|Spark Violet™ 423 anti-mouse IgM||RMM-1||FC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
Purified anti-mouse IgM
PE anti-mouse IgM
Biotin anti-mouse IgM
FITC anti-mouse IgM
APC anti-mouse IgM
PerCP/Cyanine5.5 anti-mouse IgM
APC/Cyanine7 anti-mouse IgM
Brilliant Violet 421™ anti-mouse IgM
PE/Cyanine7 anti-mouse IgM
Alexa Fluor® 488 anti-mouse IgM
Brilliant Violet 605™ anti-mouse IgM
Alexa Fluor® 647 anti-mouse IgM
Purified anti-mouse IgM (Maxpar® Ready)
PE/Dazzle™ 594 anti-mouse IgM
Brilliant Violet 510™ anti-mouse IgM
TotalSeq™-A0450 anti-mouse IgM
Brilliant Violet 711™ anti-mouse IgM
APC/Fire™ 750 anti-mouse IgM
TotalSeq™-C0450 anti-mouse IgM
PE/Cyanine5 anti-mouse IgM
TotalSeq™-B0450 anti-mouse IgM
Spark Violet™ 423 anti-mouse IgM