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Purified anti-mouse/human CD44 (Maxpar® Ready) Antibody

Pricing & Availability
Clone
IM7 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
IM7_PURE_CD44_Maxpar_Antibody_041317
Mouse splenocytes stained with 148Nd-anti-CD11b (M1/70) and 171Yb-anti-CD44 (IM7). Total viable cells are displayed in the analysis
  • IM7_PURE_CD44_Maxpar_Antibody_041317
    Mouse splenocytes stained with 148Nd-anti-CD11b (M1/70) and 171Yb-anti-CD44 (IM7). Total viable cells are displayed in the analysis
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103051 100 µg 85€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Human
Reported Reactivity
Chimpanzee, Baboon, Cynomolgus, Rhesus, Squirrel Monkey, Horse, Cow, Pig, Dog, Cat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Dexamethasone-induced myeloid leukemia M1 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
CyTOF® - Verified
Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Clone IM7 has been reported to recognize an epitope common to alloantigens and all isoforms of CD4417,18 that is located between amino acids 145 and 18620. This clone has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F). Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections6,7, complement-mediated cytotoxicity1, immunoprecipitation1,3, in vivo inhibition of DTH4,5, and spatial biology (IBEX)23,24. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 103046, 103065 - 103069).

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References
  1. Trowbridge IS, et al. 1982. Immunogenetics 15:299. (ICFC, IP, CMCD)
  2. Katoh S, et al. 1994. J. Immunol. 153:3440. (ELISA)
  3. Budd RC, et al. 1987. J. Immunol. 138:3120. (IP)
  4. Camp RL, et al. 1993. J. Exp. Med. 178:497. (Block)
  5. Weiss JM, et al. 1997. J. Cell Biol. 137:1137. (Block)
  6. Frank NY, et al. 2005. Cancer Res. 65:4320. (IHC) PubMed
  7. Cuff CA, et al. 2001. J. Clin. Invest. 108:1031. (IHC)
  8. Lee JW, et al. 2006. Nature Immunol. 8:181.
  9. Zhang N, et al. 2005. J. Immunol. 174:6967. PubMed
  10. Huabiao C, et al. 2005. J. Immunol. 175:591. PubMed
  11. Gui J, et al. 2007. Int. Immunol. 19:1201. PubMed
  12. Wang XY, et al. 2008. Blood 111:2436. PubMed
  13. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  14. Yamazaki J, et al. 2009. Blood PubMed
  15. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  16. Chen YW, et al. 2010. Mol. Cancer Ther. 9:2879. PubMed
  17. Zheng Z, et al. 1995. J. Cell. Biol. 130:485.
  18. Wiranowska M, et al. 2010. Int. J. Cancer 127:532.
  19. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  20. Sandmaier BM, et al. 1998. Blood 91:3494.
  21. Yang Y, et al. 2015. Hypertension. 65:1047. PubMed
  22. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
  23. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  24. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. van der Heide V, et al. 2022. Cell Rep. :110508. PubMed
  2. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  3. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  4. Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
  5. Oudelaar AM, et al. 2020. Nat Commun. 2.348611111. PubMed
  6. Bouchard G, et al. 2022. Cancer Res. 82:648. PubMed
RRID
AB_2562799 (BioLegend Cat. No. 103051)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.
5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
6. Liu T, et al. 2009. Biol. Direct 4:40.

Gene ID
12505 View all products for this Gene ID 960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD44 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-mouse/human CD44 IM7 FC
Biotin anti-mouse/human CD44 IM7 FC,ICC,IHC-P
FITC anti-mouse/human CD44 IM7 FC,ICC,IHC-P
PE/Cyanine5 anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 IM7 FC,IHC-F,CyTOF®,ELISA,ICC,IHC-P,IP,CMCD,Stim
Brilliant Violet 605™ anti-mouse/human CD44 IM7 FC
PE anti-mouse/human CD44 IM7 FC
Alexa Fluor® 488 anti-mouse/human CD44 IM7 FC,ICC,IHC-P,SB
Alexa Fluor® 647 anti-mouse/human CD44 IM7 FC,ICC,SB
Pacific Blue™ anti-mouse/human CD44 IM7 FC
Alexa Fluor® 700 anti-mouse/human CD44 IM7 FC,SB
PE/Cyanine7 anti-mouse/human CD44 IM7 FC
APC/Cyanine7 anti-mouse/human CD44 IM7 FC
PerCP/Cyanine5.5 anti-mouse/human CD44 IM7 FC
PerCP anti-mouse/human CD44 IM7 FC
Brilliant Violet 421™ anti-mouse/human CD44 IM7 FC,ICC,IHC-P
Brilliant Violet 570™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 785™ anti-mouse/human CD44 IM7 FC
Brilliant Violet 510™ anti-mouse/human CD44 IM7 FC
Ultra-LEAF™ Purified anti-mouse/human CD44 IM7 FC,CyTOF®,ELISA,IHC,IP,CMCD,Stim
Brilliant Violet 650™ anti-mouse/human CD44 IM7 FC
Purified anti-mouse/human CD44 (Maxpar® Ready) IM7 FC,CyTOF®
Alexa Fluor® 594 anti-mouse/human CD44 IM7 ICC,FC,IHC-P,SB
PE/Dazzle™ 594 anti-mouse/human CD44 IM7 FC
Brilliant Violet 711™ anti-mouse/human CD44 IM7 FC
APC/Fire™ 750 anti-mouse/human CD44 IM7 FC
TotalSeq™-A0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-C0073 anti-mouse/human CD44 IM7 PG
TotalSeq™-B0073 anti-mouse/human CD44 IM7 PG
Spark YG™ 570 anti-mouse/human CD44 IM7 FC,IHC-P
Spark YG™ 593 anti-mouse/human CD44 IM7 FC
TotalSeq™-D0073 anti-mouse/human CD44 IM7 PG
Brilliant Violet 750™ anti-mouse/human CD44 IM7 FC
Go To Top Version: 4    Revision Date: 07/21/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC anti-mouse/human CD44

  • Biotin anti-mouse/human CD44

  • FITC anti-mouse/human CD44

  • PE/Cyanine5 anti-mouse/human CD44

  • Purified anti-mouse/human CD44

  • Brilliant Violet 605™ anti-mouse/human CD44

  • PE anti-mouse/human CD44

  • Alexa Fluor® 488 anti-mouse/human CD44

  • Alexa Fluor® 647 anti-mouse/human CD44

  • Pacific Blue™ anti-mouse/human CD44

  • Alexa Fluor® 700 anti-mouse/human CD44

  • PE/Cyanine7 anti-mouse/human CD44

  • APC/Cyanine7 anti-mouse/human CD44

  • PerCP/Cyanine5.5 anti-mouse/human CD44

  • PerCP anti-mouse/human CD44

  • Brilliant Violet 421™ anti-mouse/human CD44

  • Brilliant Violet 570™ anti-mouse/human CD44

  • Brilliant Violet 785™ anti-mouse/human CD44

  • Brilliant Violet 510™ anti-mouse/human CD44

  • Ultra-LEAF™ Purified anti-mouse/human CD44

  • Brilliant Violet 650™ anti-mouse/human CD44

  • Purified anti-mouse/human CD44 (Maxpar® Ready)

  • Alexa Fluor® 594 anti-mouse/human CD44

  • PE/Dazzle™ 594 anti-mouse/human CD44

  • Brilliant Violet 711™ anti-mouse/human CD44

  • APC/Fire™ 750 anti-mouse/human CD44

  • TotalSeq™-A0073 anti-mouse/human CD44

  • TotalSeq™-C0073 anti-mouse/human CD44

  • TotalSeq™-B0073 anti-mouse/human CD44

  • Spark YG™ 570 anti-mouse/human CD44

  • Spark YG™ 593 anti-mouse/human CD44

  • TotalSeq™-D0073 anti-mouse/human CD44

  • Brilliant Violet 750™ anti-mouse/human CD44
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