Purified anti-IRF3 Antibody

Pricing & Availability
SL-12 (See other available formats)
Regulatory Status
Other Names
Interferon regulatory factor-3 (IRF-3)
Mouse IgG1, κ
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Product Citations
Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-human IRF-3 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
  • IRF-3_WB_031610
    Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-human IRF-3 antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
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648701 25 µg 100€
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IRF-3 is a member of the interferon regulatory transcription factor (IRF) family. It mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity. DsRNA generated during the course of a viral infection leads to IRF-3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB protein binding to dsRNA-activated factor 1, a complex which activates the transcription of genes under control of ISRE. The phosphorylated IRF-3 is under negative regulation by ubiquitin-mediated degradation. Recent research indicates that it plays a critical role in stimulated TLR-mediated IL-27 P28 gene expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Recombinant GST-IRF-3(56-427) protein
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

WB - Quality tested
IP - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

Application References
  1. Ronco LV, et al. 1998. Genes Dev. 12:2061.
AB_2126989 (BioLegend Cat. No. 648701)

Antigen Details

47 Kd protein contains DNA binding domain

Inactive IRF3 continuously transits between the cytoplasm and nucleus. Serine/threonine phosphorylation or IRF-3 results in the structural changes which allow IRF-3 activation. The activated IRF3 translocates to the nucleus, where it forms complex with CREB binding protein and activates the transcription of interferons, interferon stimulated genes and other antiviral response genes.

IRF-3 is a constitutively expressed, latent transcription factor that plays a pivotal role in type I IFN response.
CREB binding protein, IRF-7, TICAM-1, TICAM-2, non-canonical IKB kinases TBK1 and IKKε
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Lin R, et al. 1999. Mol. Cell Biol. 19:959.
2. Hiscott J, et al. 2007. 282:15325.
3. Molle C, et al. 2010. J. Immunol. 184:1784.

Gene ID
3661 View all products for this Gene ID
View information about IRF3 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 06/09/2022

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  • Purified anti-IRF3


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