Purified anti-IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
J053B5 (See other available formats)
Regulatory Status
Other Names
Epstein-Barr Virus Induced 3, EBV-Induced Gene 3 Protein, IL-27 Subunit Beta, IL-27B, IL-35 subunit
Rat IgG2a, κ
Ave. Rating
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Product Citations
Western blot analysis of 50 ng of recombinant human IL-27 (lane 1), 50 ng of recombinant mouse IL-27 (lane 2), and 50 ng of recombinant human IL-35 (lane 3) using anti-EBI3 antibody (clone J053B5).
  • J053B5_Purified_EBI3_Antibody_WB_102214
    Western blot analysis of 50 ng of recombinant human IL-27 (lane 1), 50 ng of recombinant mouse IL-27 (lane 2), and 50 ng of recombinant human IL-35 (lane 3) using anti-EBI3 antibody (clone J053B5).
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662602 100 µg 212€
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EBI3 is an IL-12 p40-related protein that can heterodimerized with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells (DCs) following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 differentiation, increase cytolytic activity of CD8+ T cells, and impact B cell differentiation and immunoglobulin production. IL-35 is also involved in tumorigenesis and is a promising biomarker for assessing tumor progression and prognosis in clinical settings.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse EBI3
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

AB_2564303 (BioLegend Cat. No. 662602)

Antigen Details

229 amino acids, predicted molecular weight of approximately 25 kD.

B cells, monocytes, and dendritic cells.

Pro- and anti-inflammatory.
IL-27 p28 subunit and IL-35 p35 subunit.
WSX-1(TCCR) and gp130.
Cell Type
B cells, Dendritic cells, Monocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Antigen References

1. Pflanz S, et al. 2002. Immunity 16:779.
2. Takeda A, et al. 2003. J. Immunol. 170:4886.
3. Pflanz S, et al. 2004. J. Immunol. 172:2225.
4. Li MF, et al. 2013. Dev. Comp. Immunol. 41:514.
5. Zeng JC, et al. 2013. Int. J. Clin. Exp. Pathol. 6:1806.
6. Ouyang H, et al. 2014. Am. J. Med. Sci. 348:156.

Gene ID
10148 View all products for this Gene ID
View information about IL-27 IL-35 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06/14/2022

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  • Purified anti-IL-27/IL-35 EBI3 (monomer, dimer, heterodimer)


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