Purified anti-human CD366 Tim-3 Maxpar Ready Antibody anti-CD366

Pricing & Availability

Clone: F38-2E2 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: T cell immunoglobulin and mucin domain containing protein 3, hepatitis virus cellular receptor 2, CD366
Isotype: Mouse IgG1, κ
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Product Citations: publications

Human PBMCs were incubated for 3 days in media alone (top) or with PHA (bottom). Cells were then stained with 145Nd-anti-CD4 (RPA-T4) and 153Eu-anti-human CD366 (Tim-3, clone F38-2E2). CD3+ T cells are displayed in the analysis. Data provided by DVS Sciences.

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345019

100 µg

207€

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Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on activated T cells (preferentially on Th1 cells, monocytes/macrophages, and dendritic cells). Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human Tim-3 fusion protein

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for relevant formats of this clone) include: costimulation¹ (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking^2,3.

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
Klibi J, et al 2009. Blood 113:1957. (FC, Block)

Product Citations

Gadalla R, et al. 2022. STAR Protoc. 3:101643. PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
Michelozzi IM, et al. 2022. STAR Protoc. 3:101174. PubMed
Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed

RRID

AB_2563790 (BioLegend Cat. No. 345019)

Antigen Details

Structure: Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
Distribution: Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
Function: Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
Ligand/Receptor: Galectin-9
Cell Type: Dendritic cells, Monocytes, T cells, Th1, Tregs
Biology Area: Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.
Gene ID: 84868 View all products for this Gene ID
UniProt: View information about CD366 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD366 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-human CD366 (Tim-3)	F38-2E2	FC
PE anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 421™ anti-human CD366 (Tim-3)	F38-2E2	FC
Ultra-LEAF™ Purified anti-human CD366 (Tim-3)	F38-2E2	FC,Costim,Block
APC anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Cyanine7 anti-human CD366 (Tim-3)	F38-2E2	FC
PerCP/Cyanine5.5 anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 605™ anti-human CD366 (Tim-3)	F38-2E2	FC
FITC anti-human CD366 (Tim-3)	F38-2E2	FC
Purified anti-human CD366 (Tim-3) (Maxpar® Ready)	F38-2E2	FC,CyTOF®
Brilliant Violet 711™ anti-human CD366 (Tim-3)	F38-2E2	FC
APC/Cyanine7 anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 785™ anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 650™ anti-human CD366 (Tim-3)	F38-2E2	FC
Brilliant Violet 510™ anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Dazzle™ 594 anti-human CD366 (Tim-3)	F38-2E2	FC
GoInVivo™ Purified anti-human CD366 (Tim-3)	F38-2E2	FC,Costim,Block
APC/Fire™ 750 anti-human CD366 (Tim-3)	F38-2E2	FC
Pacific Blue™ anti-human CD366 (Tim-3)	F38-2E2	FC
Biotin anti-human CD366 (Tim-3)	F38-2E2	FC
TotalSeq™-A0169 anti-human CD366 (Tim-3)	F38-2E2	PG
TotalSeq™-C0169 anti-human CD366 (Tim-3)	F38-2E2	PG
PE/Cyanine5 anti-human CD366 (Tim-3)	F38-2E2	FC
TotalSeq™-B0169 anti-human CD366 (Tim-3)	F38-2E2	PG
Brilliant Violet 750™ anti-human CD366 (Tim-3) Antibody	F38-2E2	FC
TotalSeq™-D0169 anti-human CD366 (Tim-3)	F38-2E2	PG
PE/Fire™ 810 anti-human CD366 (Tim-3)	F38-2E2	FC
PE/Fire™ 640 anti-human CD366 (Tim-3)	F38-2E2	FC

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 07/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.