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APC anti-mouse CD195 (CCR5) Antibody

Pricing & Availability
Clone
HM-CCR5 (See other available formats)
Regulatory Status
RUO
Other Names
CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM-CCR5_APC_090407
Mouse CCR5 transfected cells stained with HM-CCR5 APC
  • HM-CCR5_APC_090407
    Mouse CCR5 transfected cells stained with HM-CCR5 APC
Compare all formats See APC spectral data
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107011 25 µg 100€
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107012 100 µg 252€
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Description

CD195 is a 45 kD chemokine receptor also known as CCR5. CD195 is a seven transmembrane-spanning G protein-associated molecule expressed on macrophages, a T cell subset, and in the heart, liver, and spleen. CD195 regulates lymphocyte chemotaxis and transendothelial migration during inflammatory processes. CD195 interacts with several ligands including RANTES, MCP-1, MIP-1α, and MIP-1β.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

CCR5 is expressed at low density on activated cells. For successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 107006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 107004) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)).

Application References
  1. Mao A, et al. 2005. J. Immunol. 175:5146. (FC) PubMed
  2. Ishida Y, et al. 2007. Am J Pathol.170:843.(FC) PubMed
  3. Zeiser Z, et al. 2008. Blood 111:453. (FC) PubMed
  4. Sharma R, et al. 2009. J. Immunol.. 183:3212 (FC) PubMed
  5. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  2. Mirshahi F, et al. 2022. Cell Rep. 38:110454. PubMed
  3. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  4. Zhu X et al. 2019. Cell reports. 27(4):1205-1220 . PubMed
  5. Galle-Treger L, et al. 2020. J Allergy Clin Immunol. 145:502. PubMed
  6. Moro T, et al. 2016. PLoS One. 11: 0146592. PubMed
  7. Lind L, et al. 2021. J Neurovirol. 27:145. PubMed
  8. Ulaganathan VK, et al. 2020. Sci Rep. 10:8453. PubMed
  9. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  10. Schnoegl D, et al. 2022. Front Immunol. 13:909270. PubMed
  11. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  12. Kong YY, et al. 2020. Vaccines (Basel). 8:00. PubMed
RRID
AB_2074528 (BioLegend Cat. No. 107011)
AB_2074527 (BioLegend Cat. No. 107012)

Antigen Details

Structure
β-chemokine receptor, 45 kD
Distribution

Macrophages, T cell subset, heart, spleen, liver

Function
Lymphocyte chemotaxis and transendothelial migration during inflammation, signaling through seven transmembrane-spanning G proteins
Ligand/Receptor
RANTES, MCP-1, MIP-1α, and MIP-1β
Cell Type
Dendritic cells, Macrophages, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Napolitano M, et al. 1990. J. Exp. Med. 172:285.
3. Meyer A, et al. 1996. J. Biol. Chem. 271:14445.
4. Boring, et al. 1996. J. Biol. Chem. 271:7551.

Gene ID
12774 View all products for this Gene ID
UniProt
View information about CD195 on UniProt.org

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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