Using the right buffers is critical for assay performance and reliable results. Our solutions are optimized to support your experiments in a variety of applications. Find the optimal buffers to complement your experiments, either as preassembled kits or individual reagents.


Flow Cytometry

Discover our buffers optimized to help you fix, stain, permeate, or detect proteins in your flow cytometry experiments.

View all FC buffers >


Confidently execute imaging experiments with buffers to clear tissue, improve antibody binding, or boost fluorescent signal.

View all microscopy buffers >


Use our ELISA-optimized buffers to coat, wash and block plates for ideal binding and ultimate assay performance.

View all immunoassay buffers >


Confidently execute our bead-based immunoassays by pairing with the right LEGENDplex™ buffer set.

View all LEGENDplex™ buffers >

Western Blotting

Trust our Western-Ready™ buffers for every step of your workflow, from protein extraction to detection.

View all WB buffers >


Achieve peak performance and reduce non-specific binding in your magnetic cell separation system using our buffer.

View the MojoSort™ buffer >

Selecting the Right Buffers for Flow Cytometry


Beyond antibody reagents, flow cytometry requires the right types of buffers for optimal staining. This convenient list separates out flow cytometry applications by their intended target. It also indicates which buffers are best-suited to your task for surface or intracellular staining and the protocols necessary for each.


Additionally, our Tandem Stabilizer helps to reduce tandem dye de-coupling when added as a supplement to our commonly used buffers for storing stained, fixed cells, as well as those used for fixation and permeabilization procedures. 

Equivalent Products


BD Biosciences

420403 7-AAD Viability Staining Solution 559925 7-AAD Staining Solution
421301 Propidium Iodide 556463 Propidium Iodide
420701 Monensin Solution (1,000X) 554724 BD™ GolgiStop
420601 Brefeldin A 555029 BD™ GolgiPlug
422201 Annexin V Binding Buffer 556454 Annexin V Binding Buffer
420301 RBC Lysis Buffer (10X) 555899 Lysing Buffer
422401 RBC Lysis/Fixation Solution (10X) 558049 Lyse/Fix Buffer 5X
156603 TruStain FcX™ PLUS (Mouse) 553141 Mouse Fc Block
422301 TruStain FcX™ (Human) 564219 BD Fc Block™
420201 Cell Staining Buffer 554656 Stain Buffer (FBS)
420801 Fixation Buffer 554722 BD Cytofix/Cytoperm™ Solution
421002 Intracellular Staining Permeabilization Wash Buffer (10X) 554723 Perm/Wash Buffer
424401 True-Nuclear™ Transcription Factor Buffer Set 560409/560098 (Mouse/Human) FOXP3 Buffer Set
422101 FluoroFix™ Buffer 338036 BD™ Stabilizing Fixative
422501 Cyto-Last™ Buffer None
424401 True-Nuclear™ Transcription Factor Buffer Set 562574 Transcription Factor Buffer Set
425401 True-Phos™ Perm Buffer 558050 BD Phosflow™ Perm Buffer III
426101 True-Stain Monocyte Blocker™ None
426803 Cyto-Fast™ Fix/Perm Buffer Set 554714 Fixation/Permeabilization Solution Kit

Eliminate Monocyte Non-Specific Antibody Binding in Flow Cytometry


Monocytes and macrophages can exhibit non-specific binding of antibodies and fluorophores used in cell surface staining of live cells. BioLegend has now formulated an effective blocking reagent, True-Stain Monocyte Blocker™. This non-antibody based blocking solution has been shown to reduce non-specific monocyte binding due to the fluorophore and does not affect the desirable specific staining of monocytes. Learn more about how True-Stain Monocyte Blocker™ can help you immuno-label monocytes with confidence.

True-Stain Monocyte Blocker™ prevents monocytes from non-specifically binding to fluorophores

Human PBMCs were stained with 5 µL/test of antibody in 100 µL of cells at 1 x 106 cells/mL with (bottom row)

or without (top row) True-Stain Monocyte Blocker™.

Buffers to Support your Western Blotting Experiments


Our Western-Ready™ buffers are easily integrated into your workflow to support protein extraction, gel loading and running, and detection. Explore our Western blot page to learn more about our buffers, protocols and troubleshooting tips!


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