Purified anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
RMP1-14 (See other available formats)
Regulatory Status
Other Names
Programmed Death-1, CD279, PD 1
Rat IgG2a, κ
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Product Citations
Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
  • RMP1-14_Pure_022309.jpg
    Con A-stimulated C57BL/6 splenocytes stained with purified RMP1-14, followed by biotinylated anti-rat IgG and Sav-PE
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114101 50 µg 67€
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114102 500 µg 102€
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CD279 is a 50-55 kD immunoglobulin superfamily member, also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production costimulated by macrophages (but not by dendritic cells and B cells).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse PD-1 transfected BHK cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze."

FC - Quality tested
IHC, WB, FA - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for relevant formats of this clone) include: immunohistochemical staining of acetone-fixed frozen sections1, Western blotting2,6, inhibition of macrophages (but not B cells and dendritic cells) costimulated T cell proliferation3,5, and blocking the binding of PD-1 to B7-H1 or B7-DC3,4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 114108). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 114110) with a lower endotoxin limit than standard LEAF™ purified antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Kanai, T., et al. 2003. J. Immunol. 171:4156. (FC, IHC)
  2. Personal Communication (WB)
  3. Yamazaki, T., et al. 2005. J. Immunol. 175:1586. (Costim)
  4. Matsumoto, K., et al. 2004. J. Immunol. 172:2530. (Block)
  5. Terrazas, L. I., et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
  6. Meng, Q., et al. 2006. Invest Opthalmol Vis Sci. 47:444. (FC, WB) PubMed
  7. Tsang JY, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  8. Takamura S, et al. 2010. J. Immunol. 184:4696. PubMed (Block)
Product Citations
  1. Chen Y, et al. 2018. Oncoimmunology. 7:e1388484. PubMed
  2. Chen L, et al. 2020. Front Immunol. 11:584458. PubMed
  3. Wang G, et al. 2020. Nat Commun. 11:1395. PubMed
  4. López-Medina M, et al. 2015. Immunobiology. . PubMed
  5. Xu YY, et al. 2017. J Reprod Dev. 63:289. PubMed
  6. Len-Letelier RA, et al. 2020. Frontiers in Immunology. 11:583382. PubMed
  7. Zhang H, et al. 2019. Mediators Inflamm. 2019:8492090. PubMed
  8. Tsang J, et al. 2011. Int Immunopharmacol. 11:604. PubMed
  9. Wei W, et al. 2022. mSystems. 7:e0046922. PubMed
  10. Jang SC, et al. 2021. Commun Biol. 4:497. PubMed
  11. Meng Q, et al. 2006. Invest Ophthalmol Vis Sci. 47:4444. PubMed
  12. Jiao S, et al. 2020. Cell. 179(5):1177-1190.e13.. PubMed
  13. Arana Y, et al. 2022. Front Immunol. 13:866120. PubMed
  14. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  15. Julian M, et al. 2015. PLoS One. 10: 0132921. PubMed
AB_313572 (BioLegend Cat. No. 114101)
AB_313573 (BioLegend Cat. No. 114102)

Antigen Details

Ig superfamily, 50-55 kD

Subset of double negative thymocytes, activated T and B cells

Lymphocyte clonal selection, peripheral tolerance
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook, Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
View information about CD279 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 02/17/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD279 (PD-1)

  • LEAF™ Purified anti-mouse CD279 (PD-1)

  • Ultra-LEAF™ Purified anti-mouse CD279 (PD-1)

  • GoInVivo™ Purified anti-mouse CD279 (PD-1)

  • PE anti-mouse CD279 (PD-1)


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