Purified anti-mouse CD14 Maxpar Ready Antibody anti-CD14

Pricing & Availability

Clone: Sa14-2 (See other available formats)
Regulatory Status: RUO
Other Names: LPS receptor
Isotype: Rat IgG2a, κ
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Product Citations: publications

MC/9 (top) or J774A.1 (bottom) mouse cell lines stained with 156Gd anti-CD14 (Sa14-2). Data provided by DVS Sciences.

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123321

100 µg

95€

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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed on macrophages, dendritic cells, Kupffer cells, hepatocytes, and granulocytes. As a high-affinity receptor for LPS-LBP (LPS-binding protein) complex, CD14, in association with Toll-like Receptor 4 (TLR4) or 2 (TLR2), is involved in the clearance of gram-negative pathogens.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
RRID: AB_2563720 (BioLegend Cat. No. 123321)

Antigen Details

Structure: GPI-linked membrane glycoprotein, 53-55 kD
Distribution: macrophages, dendritic cells, kupffer cells, hepatocytes
Function: LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor: LPS
Cell Type: Dendritic cells, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Akashi S, et al. 2003. J. Exp. Med. 198:1035.
3. Matsuura K, et al. 1994. J. Exp. Med. 179:1671.
4. Liu S, et al. 1998. Infect. Immun. 66:5089.
Gene ID: 12475 View all products for this Gene ID
UniProt: View information about CD14 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

View All CD14 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-mouse CD14	Sa14-2	FC
Biotin anti-mouse CD14	Sa14-2	FC
FITC anti-mouse CD14	Sa14-2	FC
PE anti-mouse CD14	Sa14-2	FC
APC anti-mouse CD14	Sa14-2	FC
PerCP/Cyanine5.5 anti-mouse CD14	Sa14-2	FC
PE/Cyanine7 anti-mouse CD14	Sa14-2	FC
APC/Cyanine7 anti-mouse CD14	Sa14-2	FC
Purified anti-mouse CD14 (Maxpar® Ready)	Sa14-2	FC,CyTOF®
Brilliant Violet 510™ anti-mouse CD14	Sa14-2	FC
Purified anti-mouse CD14	Sa14-2	WB, IF, FC
Brilliant Violet 421™ anti-mouse CD14	Sa14-2	FC
Alexa Fluor® 647 anti-mouse CD14	Sa14-2	FC
PE/Dazzle™ 594 anti-mouse CD14	Sa14-2	FC
APC/Fire™ 750 anti-mouse CD14	Sa14-2	FC
TotalSeq™-A0424 anti-mouse CD14	Sa14-2	PG
TotalSeq™-C0424 anti-mouse CD14	Sa14-2	PG
TotalSeq™-B0424 anti-mouse CD14	Sa14-2	PG
Brilliant Violet 605™ anti-mouse CD14	Sa14-2	FC
Brilliant Violet 785™ anti-mouse CD14 Antibody	Sa14-2	FC

Concentration & Expiration Lookup

Certificate of Analysis

Go To Top Version: 2 Revision Date: 07/21/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.