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PerCP/Cyanine5.5 anti-human CD31 Antibody

Pricing & Availability
Clone
WM59 (See other available formats)
Regulatory Status
RUO
Workshop
V P025
Other Names
PECAM-1, EndoCAM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM59_PerCPCyanine55_CD31_Antibody_110718
Human peripheral blood granulocytes were stained with CD31 (clone WM59) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram)
  • WM59_PerCPCyanine55_CD31_Antibody_110718
    Human peripheral blood granulocytes were stained with CD31 (clone WM59) PerCP/Cyanine5.5 (filled histogram) or mouse IgG1, κ PerCP/Cyanine5.5 isotype control (open histogram)
Compare all formats See PerCP/Cyanine5.5 spectral data
Cat # Size Price Quantity Check Availability Save
303131 25 tests 141€
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303132 100 tests 306€
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Description

CD31 is a 130-140 kD type I transmembrane glycoprotein also known as platelet endothelial cell adhesion molecule-1 (PECAM-1) or Endocam. It is expressed on monocytes, platelets, granulocytes, endothelial cells and lymphocyte subsets. CD31 has been reported to bind CD38 and be involved in wound healing, angiogenesis, and cellular migration in an inflammatory situation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone WM59 has been reported to recognize the D2 extracellular portion of CD31.

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, immunohistochemical staining of acetone-fixed frozen tissue sections8, blocking of platelet aggregation3, and spatial biology (IBEX)11,12. Clone WM59 is not recommended for immunohistochemical staining of formalin-fixed paraffin-embedded sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 303143 & 303144).

The purified WM59 antibody is useful as a capture antibody for a sandwich ELISA assay, when used in conjunction with biotin anti-human CD31 antibody (Cat. No. 536604) antibody as the detection antibody.

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V Oxford University Press. New York.
  2. Muczynski KA, et al. 2003. J. Am. Soc. Nephrol. 14:1336. (IF)
  3. Wu XW, et al. 1997. Arterioscl. Throm. Vas. 17:3154. (Block)
  4. Nagano M, et al. 2007. Blood 110:151. (FC) PubMed
  5. MacFadyen JR, et al. 2005. FEBS Lett. 579:2569. PubMed
  6. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  7. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  8. Wicki A, et al. 2012. Clin. Cancer Res. 18:454. (FC, IHC) PubMed
  9. Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
  10. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110 (IF) PubMed
  11. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  12. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Yu XX, et al. 2021. Cell Res. 31:886. PubMed
  2. Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed
  3. Shi X, et al. 2022. Clin Transl Med. 12:e711. PubMed
  4. Still C 2nd, et al. 2021. Cell Reports Medicine. 2(7):100343. PubMed
  5. Ramond C et al. 2017. eLife. 6 pii: e27564. PubMed
  6. Kwiecien I, et al. 2022. Front Immunol. 13:864497. PubMed
RRID
AB_2566174 (BioLegend Cat. No. 303131)
AB_2566175 (BioLegend Cat. No. 303132)

Antigen Details

Structure
Ig superfamily, type I transmembrane glycoprotein, 130-140 kD
Distribution

Monocytes, platelets, granulocytes, endothelial cells, lymphocyte subset

Function
Cell adhesion, signal transduction
Ligand/Receptor
CD38
Cell Type
Endothelial cells, Granulocytes, Lymphocytes, Monocytes, Neutrophils, Platelets
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. DeLisser H, et al. 1994. Immunol. Today 15:490.
  2. Newman P, 1997. J. Clin. Invest. 99:3.
  3. Fawcett J, et al. 1995. J. Cell Biol. 128:1229.
Gene ID
5175 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 11/07/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • FITC anti-human CD31

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  • Purified anti-human CD31

  • Alexa Fluor® 488 anti-human CD31

  • Alexa Fluor® 647 anti-human CD31

  • Pacific Blue™ anti-human CD31

  • APC anti-human CD31

  • PE/Cyanine7 anti-human CD31

  • APC/Cyanine7 anti-human CD31

  • Brilliant Violet 605™ anti-human CD31

  • Brilliant Violet 421™ anti-human CD31

  • Purified anti-human CD31 (Maxpar® Ready)

  • Alexa Fluor® 594 anti-human CD31

  • PE/Dazzle™ 594 anti-human CD31

  • PerCP/Cyanine5.5 anti-human CD31

  • Alexa Fluor® 700 anti-human CD31

  • Brilliant Violet 711™ anti-human CD31

  • TotalSeq™-A0124 anti-human CD31

  • TotalSeq™-C0124 anti-human CD31

  • APC/Fire™ 750 anti-human CD31

  • Ultra-LEAF™ Purified anti-human CD31

  • TotalSeq™-B0124 anti-human CD31

  • Brilliant Violet 785™ anti-human CD31

  • PE/Cyanine5 anti-human CD31

  • TotalSeq™-D0124 anti-human CD31

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