PE/Cyanine7 anti-mouse CD138 (Syndecan-1) Antibody

Pricing & Availability
Clone
281-2 (See other available formats)
Regulatory Status
RUO
Other Names
Syndecan-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
281-2_PECy7_CD138_Antibody_FC_1_060413
C57BL/6 mouse splenocytes were stained with CD19 APC and CD138 (clone 281-2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • 281-2_PECy7_CD138_Antibody_FC_1_060413
    C57BL/6 mouse splenocytes were stained with CD19 APC and CD138 (clone 281-2) PE/Cyanine7 (top) or rat IgG2a, κ PE/Cyanine7 isotype control (bottom).
  • 281-2_PECy7_CD138_Antibody_FC_2_060413
Compare all formats See PE/Cyanine7 spectral data
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142513 25 µg 141€
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142514 100 µg 306€
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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant plasma cells, pre-B cells, mesenchymal cells, epithelial cells, and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse mammary gland epithelial cell line NMuMG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue3 and formalin-fixed paraffin embedded tissue4 and immunofluorescent staining2,3.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Jalkanen M, et al. 1985. J. Cell. Biol. 101:976. (FC)
  2. Miettinen H, et al. 1994. J. Cell. Sci. 107:1571. (IF)
  3. Li Q, et al. 2002. Cell 111:635. (IF, IHC)
  4. McCarthy BA, et al. 2012. BMC Cancer. 12:203. (IHC)
Product Citations
  1. Wu J et al. 2017. Immunity. 47(6):1114-1128 . PubMed
  2. Xu Y, et al. 2022. Nat Commun. 13:980. PubMed
  3. Tan J, et al. 2021. iScience. 24(8):102835. PubMed
  4. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  5. Kim CJ, et al. 2018. Immunity. 49:1034. PubMed
  6. Baranek T, et al. 2020. Cell Reports. 32(10):108116. PubMed
  7. Franks SE, et al. 2019. J Immunol. 202:3381. PubMed
  8. Casali P, et al. 2021. Front Immunol. 12:761450. PubMed
  9. Chen JS, et al. 2022. Sci Immunol. 7:eabl5652. PubMed
  10. Yewdell WT, et al. 2020. Immunity. 53(5):952-970.e11. PubMed
  11. Liu X, et al. 2022. Nat Cancer. :. PubMed
  12. Zbesko JC, et al. 2021. Brain Behav Immun. 91:578. PubMed
  13. Glaros V, et al. 2021. Immunity. 54:2005. PubMed
  14. Venturutti L, et al. 2020. Cell. 182(2):297-316.e27. PubMed
  15. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  16. Fu G, et al. 2021. Nature. 595:724. PubMed
  17. Noviski M, et al. 2018. Elife. 7:e35074. PubMed
  18. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  19. Wittner J, et al. 2022. Mucosal Immunol. 15:668. PubMed
  20. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  21. Cuenca M, et al. 2016. J Immunol. 196: 726 - 737. PubMed
  22. Bello A, et al. 2021. Immunol Cell Biol. 99:879. PubMed
  23. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  24. Haniuda K, et al. 2020. Cell Rep. 33:108333. PubMed
  25. Flümann R, et al. 2021. Cancer Discov. 2:70. PubMed
  26. New JS, et al. 2020. Immunity. 53(1):172-186.e6. PubMed
  27. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  28. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  29. Flümann R, et al. 2021. Blood Cancer Discov. 2:70. PubMed
  30. Becktel DA, et al. 2021. J Neurosci. Online ahead of print. PubMed
RRID
AB_2562197 (BioLegend Cat. No. 142513)
AB_2562198 (BioLegend Cat. No. 142514)

Antigen Details

Structure
Transmembrane heparan sulfate proteoglycan; member of the syndecan proteoglycan family, 100-200 kD
Function
Cell adhesion, cell motility, cell migration, cell proliferation
Cell Type
B cells
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zong F, et al. 2011. PLoS ONE 6:e14816.
2. Yamashita Y, et al. 1999. J. Immunol. 162:5940.
3. Sanderson RD, et al. 1989. Cell. Regul. 1:27.

Gene ID
20969 View all products for this Gene ID
UniProt
View information about CD138 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 06/12/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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