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PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_AD2_PECy7_CD73_Antibody_FC_1_091712
Human peripheral blood lymphocytes were stained with CD3 Pacific Blue™ and CD73 (clone AD2) PE/Cyanine7 (top) or mouse IgG1, κ PE/Cyanine7 isotype control (bottom).
  • A_AD2_PECy7_CD73_Antibody_FC_1_091712
    Human peripheral blood lymphocytes were stained with CD3 Pacific Blue™ and CD73 (clone AD2) PE/Cyanine7 (top) or mouse IgG1, κ PE/Cyanine7 isotype control (bottom).
  • B_AD2_PECy7_CD73_Antibody_FC_2_091712
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
344009 25 tests 109€
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344010 100 tests 278€
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Slamecka J, et al. 2017. Cell Cycle. 17:330. PubMed
  2. Tanbakooei S, et al. 2022. J Reprod Infertil. 22:241. PubMed
  3. Lindner JM, et al. 2019. Immunity. 50:668. PubMed
  4. Li Y, et al. 2020. Front Cell Dev Biol. 8:604021. PubMed
RRID
AB_2561541 (BioLegend Cat. No. 344009)
AB_2561542 (BioLegend Cat. No. 344010)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 04/09/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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