Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase) Antibody

Pricing & Availability
Clone
AD2 (See other available formats)
Regulatory Status
RUO
Workshop
V B-CD73.3
Other Names
Ecto-5'-nucleotidase, E.C3.1.3.5, L-VAP-2, NT5E, 5'-NT
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_AD2_BV421_1_072111
Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Brilliant Violet 421™ or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • A_AD2_BV421_1_072111
    Human peripheral blood lymphocytes were stained with CD3 FITC and CD73 (clone AD2) Brilliant Violet 421™ or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • AD2_BV421_CD73_Antibody_012022
    Frozen human tonsil section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 10 µg/ml of Brilliant Violet 421™ anti-human CD73 (clone AD2) (blue), 10 µg/ml of Alexa Fluor® 647 anti-human CD19 (clone HIB19) (red) and 10 µg/ml of Alexa Fluor® 488 anti-human CD3 (clone UCHT1) overnight at 4°C. The image was captured by 10X objective.
  • B_AD2_BV421_2_072111
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
344007 25 tests 152€
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344008 100 tests 305€
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Description

CD73 is a 70 kD glycophosphatidylinositol (GPI)-linked 5'-nucleotidase, which is also known as ecto-5'-nucleotidase. It converts adenosine monophosphate (AMP) to adenosine. CD73 is expressed on subsets of T and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells. It has been reported that CD73 costimulates T cell activation, and mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemical staining on frozen tissue sections, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include:immunofluorescence3.

Clone AD2 has been noted to induce clustering and internalization of CD73 in vivo and inhibit metastasis in a murine breast cancer xenograft model4.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakamura T, et al. 1993. J. Immunol. 151:6933.
  2. Liao J, et al. 2011. J Endod. 37:1217. PubMed
  3. Touboul C, et al. 2013. J. Transl. Med. 11:28. (IF)
  4. Terp MG, et al. 2013. J Immunol. 191: 4165-73 (Block)
Product Citations
  1. Kaneko N, et al. 2020. Cell. 183(1):143-157.e13. PubMed
  2. Harvestine JN, et al. 2018. J Mater Chem B. 6:4104. PubMed
  3. Ehlers L, et al. 2021. The FASEB Journal. 35(7):e21684. PubMed
  4. Ng E, et al. 2016. Nat Biotechnol. 34:1168-1179. PubMed
  5. Harvestine JN, et al. 2020. Sci Adv. 6:eaay2387. PubMed
  6. Hazenberg MD, et al. 2019. Blood Adv. 2.659722222. PubMed
  7. Eikmans M, et al. 2022. Front Immunol. 13:814019. PubMed
  8. Yang YK, et al. 2021. Stem Cell Res Ther. 12:156. PubMed
RRID
AB_10900981 (BioLegend Cat. No. 344007)
AB_11204424 (BioLegend Cat. No. 344008)

Antigen Details

Structure
GPI-linked 5'-nucleotidase, 70 kD
Distribution

Subsets of T cells and B cells, mesenchymal stem cells, follicular dendritic cells, endothelial cells, and epithelial cells

Function
Catalyses dephosphorylation of adenosine monophosphate, costimulates T cell activation, mediates adhesion of lymphocytes to follicular dendritic cells and endothelial cells
Cell Type
B cells, Dendritic cells, Endothelial cells, Epithelial cells, Mesenchymal Stem Cells, T cells, Tregs
Biology Area
Costimulatory Molecules, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and stromal Cell Molecules:the CD Markers. A John Wiley & Sons Inc, Publication.
2. Airas L and Jalkanen  S, et al. 1996. Blood 88:1755.
3. Gutensohn W, et al. 1995. Cell Immunol. 161:213.
4. Airas L, et al. 1995. J. Exp. Med. 182:1603.

Gene ID
4907 View all products for this Gene ID
UniProt
View information about CD73 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All CD73 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 421™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F
Purified anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC,IHC-F,Block
PE anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Pacific Blue™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Cyanine5.5 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Biotin anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PE/Dazzle™ 594 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
APC/Cyanine7 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 605™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 711™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 785™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-A0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-C0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
TotalSeq™-B0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
APC/Fire™ 750 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
TotalSeq™-D0577 anti-human CD73 (Ecto-5'-nucleotidase) AD2 PG
Alexa Fluor® 700 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Alexa Fluor® 647 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Brilliant Violet 510™ anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
PerCP/Fire™ 780 anti-human CD73 (Ecto-5'-nucleotidase) AD2 FC
Spark Red™ 718 anti-hu CD73 (Ecto-5'-nucleotidase) (Flexi-Fluor™) AD2 FC
Go To Top Version: 2    Revision Date: 04/09/2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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