Pricing & Availability
Regulatory Status
Other Names
IL-22 Pre-coated ELISA Kit
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Product Citations
mouse il-22_021810
  • mouse il-22_021810
  • mouse il-22_082718
    Naïve CD4 T cells were harvested from the spleens of Il22+/+ or Il22-/- mice (C57BL/6 background, see Immunity. 2007 Oct; 27(4):647-59).

    Cells were then cultured with plate-bound aCD3 and aCD28 mAbs in IMDM (Th0 Conditions); or additionally with recombinant mouse IL-23 (50 ng/ml), IL-1b (20 ng/ml), IL-6 (20 ng/ml) and 200 nM 6-formylindolo(3,2-b)carbazole (Th22 Conditions).

    Three days post-stimulation, supernatants were harvested, and IL-22 was quantitated using the LEGEND MAX™ Mouse IL-22 ELISA Kit (Cat. No. 436307).

    Data were provided courtesy of Prof. Lauren A. Zenewicz, University of Oklahoma.
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436307 1 Pre-coated Plate 328€
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IL-22 belongs to a family of cytokines structurally related to IL-10, including IL-19, IL-20, IL-24, and IL-26. Mouse IL-22 shares 79% amino acid identity with human IL-22. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of the T helper 17 lineage of CD4+ effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in the disregulated inflammation observed in autoimmune diseases.

BioLegend’s LEGEND MAX™ Mouse IL-22 ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Mouse IL-22 specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Mouse IL-22 ELISA Kit is specifically designed for the accurate quantitation of Mouse IL-22 from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical Data Sheet (pdf)

Kit Contents

Kit Contents
  • Anti-Mouse IL-22 Pre-coated 96-well Strip Microplate
  • Mouse IL-22 Dectection Antibody
  • Mouse IL-22 Standard
  • Avidin-HRP A
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution F
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Product Citations
  1. Mukherjee P, et al. 2022. J Lipid Res. 63:100153. PubMed
  2. Ricardo-Gonzalez RR, et al. 2022. Immunity. :. PubMed
  3. Li Y, et al. 2018. Cell Mol Immunol. 15:518. PubMed
  4. Zhu Y, et al. 2021. EMBO J. 40:e105320. PubMed
1.6 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Th17, Th1, Th2, Th22, lymphoid tissue inducer cells (LTi), and subsets of natural killer cells
Biology Area
Immunology, Stem Cells
Molecular Family
Gene ID
50929 View all products for this Gene ID
View information about IL-22 on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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