FITC anti-mouse TCR Vα8.3 Antibody

Pricing & Availability
Clone
B21.14 (See other available formats)
Regulatory Status
RUO
Other Names
T cell receptor Vα 8.3
Isotype
Rat IgG1, κ
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Product Citations
publications
B21dot14_FITC_070808.jpg
C57BL/6 splenocytes stained with CD3 (145-2C11) PE and B21.14 FITC
  • B21dot14_FITC_070808.jpg
    C57BL/6 splenocytes stained with CD3 (145-2C11) PE and B21.14 FITC
Compare all formats See FITC spectral data
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127705 50 µg 62€
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Description

The B21.14 antibody recognizes an epitope of the CDR1 on the Va 8.3 subfamily of TCR having the a,b,c and d haplotypes of Tcra gene complex.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Soluble TCR from mouse CTL clone CW3/1.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Necker A, et al. 1991. Eur J. Immunol. 21:3035
  2. Brodnicki TC, et al. 1996. Mol Immunol 33:253
  3. Tomonari K, et al. 1992. Immunogenetics 35:291
RRID
AB_1186078 (BioLegend Cat. No. 127705)

Antigen Details

Structure
TCR has a two chain structure, consisting of alpha and beta chain. It belongs to Ig superfamily.
Distribution

On a subset of T cells

Function
Recognize MHC/peptide complex on the antigen-presenting cells.
Interaction
Associate with CD3 complex to form TCR complexes expressing on cell surface.
Ligand/Receptor
TCR recognizes MHC/peptide complex
Cell Type
T cells
Biology Area
Adaptive Immunity, Immunology
Molecular Family
TCRs
Antigen References

1. Kubo RT, et al. 1989. J. Immunol. 142:2736
2. Brodnicki TC, et al. 1996. Mol Immunol 33:253

Gene ID
110469 View all products for this Gene ID
UniProt
View information about TCR Valpha8.3 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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