APC/Fire™ 810 anti-human CD223 (LAG-3) Antibody

Pricing & Availability
11C3C65 (See other available formats)
Regulatory Status
Other Names
CD223, LAG-3, LAG3, lymphocyte-activation gene-3
Mouse IgG1, κ
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Product Citations
CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells were stained with anti-human CD8 FITC and anti-human CD223 (LAG-3) (clone 11C3C65) APC Fire™ 810 (left) or mouse IgG1, κ APC Fire™ 810 isotype control (right).
  • 11C3C65_APC-Fire-810_CD223_Antibody_051622
    CD3/CD28/IL-2 stimulated (three days) peripheral blood mononuclear cells were stained with anti-human CD8 FITC and anti-human CD223 (LAG-3) (clone 11C3C65) APC Fire™ 810 (left) or mouse IgG1, κ APC Fire™ 810 isotype control (right).
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369349 25 tests 160€
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369350 100 tests 352€
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CD223, also known as LAG-3, is a 70 kD type I transmembrane glycoprotein that is involved in T-cell signaling. Similar to CD4, CD223 binds MHC class II, but with a higher affinity. CD223 negatively regulates T-cell activation. It is expressed by activated T-cells and natural killer cells (NKs), as well as regulatory T-cells. It is transiently expressed on the surface of activated T-cells in acute conditions but high expression is maintained under tolerizing conditions. CD223 deficiency results in reduced tumor growth. CD223 and PD-1 can act in synergy and reverse exhausted phenotypes, improve tumor rejection, and control viral load.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Human LAG-3 transfected cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.

Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Red Laser (633 nm)
Application Notes

The staining of clone 11C3C65 cannot be blocked by clone 7H2C65, which is another anti-human CD223 (LAG-3) antibody.

AB_2922586 (BioLegend Cat. No. 369349)
AB_2922586 (BioLegend Cat. No. 369350)

Antigen Details

70 kD transmembrane glycoprotein, Ig superfamily, highly homologous to CD4.

Activated T-cells and natural killer cells (NKs) and regulatory T cells

Negatively regulates T-cell activation.
Binds MHC class II molecules
Cell Type
Dendritic cells, NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References
  1. Castelli C, et al. 2014. Oncoimmunology. 3:e967146.
  2. Poirier N, et al. 2011. Clin Exp Immunol. 164:265.
  3. Juno JA, et al. 2015. Retrovirology. 12:17.
  4. Casati C, et al. 2006. Cancer Res. 66:4450.
Gene ID
3902 View all products for this Gene ID
View information about CD223 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/13/2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • APC/Fire™ 810 anti-human CD223 (LAG-3)


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