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Ultra-LEAF™ Purified anti-mouse CD223 (LAG-3) Antibody

Pricing & Availability
Clone
C9B7W (See other available formats)
Regulatory Status
RUO
Other Names
LAG3, LAG-3
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
C9B7W_PURE_CD223_Antibody_1_091719
ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained with CD3ε (clone 145-2C11) APC and CD223 (LAG-3) (clone C9B7W) Purified (left) or rat IgG1, κ Purified isotype control (right) followed by anti-Rat IgG PE.
  • C9B7W_PURE_CD223_Antibody_1_091719
    ConA + IL-2 stimulated (3 days) C57BL/6 splenocytes stained with CD3ε (clone 145-2C11) APC and CD223 (LAG-3) (clone C9B7W) Purified (left) or rat IgG1, κ Purified isotype control (right) followed by anti-Rat IgG PE.
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125235 50 mg 1884€
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125236 100 mg 2707€
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125234 25 mg 1127€
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125231 100 µg 88€
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125232 1 mg 120€
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125233 5 mg 432€
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This product is eligible for a 40% discount! Promotion valid until December 31, 2023. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD223, also known as LAG-3, is 70 kD type I transmembrane glycoprotein within the Ig superfamily. It is the gene product of lymphocyte activation gene-3 (Lag3) with structural similarity to CD4. CD223 is expressed on activated T cells and NK cells, as well as T regulatory cells. CD223, like CD4, binds MHC class II molecules, but with higher affinity than CD4. In association with the CD3 complex, CD223 has been shown as a negative regulatory molecule to inhibit TCR-mediated signaling and regulate homeostatic T cell expansion. In contrast to inhibition, CD223 has an activation function to promote immune responses by inducing MHC class II signaling in antigen presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

FC - Quality tested
FA, IP, ELISA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Workman CJ, et al. 2002. Eur. Immunol. 32:2255. (IP, ELISA)
  2. Workman CJ, et al. 2005. J. Immunol. 174:688. (FC)
  3. Li N, et al. 2004. J. Immunol. 173:6806. (IP, ELISA)
RRID
AB_2814003 (BioLegend Cat. No. 125235)
AB_2814004 (BioLegend Cat. No. 125236)
AB_2814002 (BioLegend Cat. No. 125234)
AB_2813999 (BioLegend Cat. No. 125231)
AB_2814000 (BioLegend Cat. No. 125232)
AB_2814001 (BioLegend Cat. No. 125233)

Antigen Details

Structure
Ig superfamily
Distribution

T cells and NK cells, as well as T regulatory cells

Cell Type
NK cells, T cells, Tregs
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Baixeras E et al. 1992. J. Exp. Med. 176:327.

Gene ID
16768 View all products for this Gene ID
UniProt
View information about CD223 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 09/12/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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