APC/Cyanine7 anti-human CD279 (PD-1) Antibody

Pricing & Availability
Clone
NAT105 (See other available formats)
Regulatory Status
RUO
Other Names
PD1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
NAT105_APCCyanine7_CD279_Antibody_010819_updated.png
PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD279 (clone NAT105) APC/Cyanine7 (filled histogram) or Mouse IgG1, ? APC/Cyanine7 isotype control (open histogram)
  • NAT105_APCCyanine7_CD279_Antibody_010819_updated.png
    PHA-stimulated (3-day) human peripheral blood lymphocytes were stained with CD279 (clone NAT105) APC/Cyanine7 (filled histogram) or Mouse IgG1, ? APC/Cyanine7 isotype control (open histogram)
Compare all formats See APC/Cyanine7 spectral data
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367415 25 tests 120€
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367416 100 tests 278€
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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune diseases. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. The interaction with CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
YT cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats of this clone) include: immunofluorescence staining.

We recommend using our Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB) (Cat. No. 929501).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Roncador G, et al. 2007. Haematologica 92:1059.
  2. Nam-Cha SH, et al. 2008. Am. J. Surg. Pathol. 32:1252.
  3. Rodriguez-Pinilla SM, et al. 2009. Am. J. Surg. Pathol. 33:81.
Product Citations
  1. Sekine T, et al. 2020. Cell. 183:158. PubMed
RRID
AB_2616743 (BioLegend Cat. No. 367415)
AB_2616744 (BioLegend Cat. No. 367416)

Antigen Details

Structure
55 kD type I transmembrane protein.
Distribution

Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.

Function
Signaling, co-stimulation (co-inhibition), immunoglobulin superfamily.
Interaction
SHP-1 and SHP-2.
Ligand/Receptor
PD-L1 (CD274) and PD-L2 (CD273).
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Cancer Biomarkers, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Francisco LM, Sage PT, and Sharpe AH. 2010. Immunological Rev. 236:219.

Gene ID
5133 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 01/08/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD279 (PD-1)

  • PE anti-human CD279 (PD-1)

  • APC anti-human CD279 (PD-1)

  • Alexa Fluor® 488 anti-human CD279 (PD-1)

  • PerCP/Cyanine5.5 anti-human CD279 (PD-1)

  • PE/Cyanine7 anti-human CD279 (PD-1)

  • FITC anti-human CD279 (PD-1)

  • APC/Cyanine7 anti-human CD279 (PD-1)

  • Alexa Fluor® 647 anti-human CD279 (PD-1)

  • Biotin anti-human CD279 (PD-1)

  • Brilliant Violet 510™ anti-human CD279 (PD-1)

  • Brilliant Violet 421™ anti-human CD279 (PD-1)

  • Brilliant Violet 711™ anti-human CD279 (PD-1)

  • Brilliant Violet 785™ anti-human CD279 (PD-1)

  • Brilliant Violet 605™ anti-human CD279 (PD-1)

  • Brilliant Violet 650™ anti-human CD279 (PD-1)

  • PE/Dazzle™ 594 anti-human CD279 (PD-1)

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