APC/Cyanine7 anti-human CD235a (Glycophorin A) Antibody

Pricing & Availability
HI264 (See other available formats)
Regulatory Status
VII 70312
Other Names
PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group, GYPA
Mouse IgG2a, κ
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Product Citations
Human red blood cells were stained with anti-human CD235a (clone HI264) APC/Cyanine7 (filled histogram) or mouse IgG2a, ? APC/Cyanine7 isotype control (open histogram).
  • HI264_APCCy7_CD235a_Antibody_040317
    Human red blood cells were stained with anti-human CD235a (clone HI264) APC/Cyanine7 (filled histogram) or mouse IgG2a, ? APC/Cyanine7 isotype control (open histogram).
Compare all formats See APC/Cyanine7 spectral data
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349115 25 tests 141€
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349116 100 tests 315€
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CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations
  1. Liu Y, et al. 2022. Signal Transduct Target Ther. 7:347. PubMed
  2. Cassandras M, et al. 2020. Nat Cell Biol. 1295:22. PubMed
  3. Caielli S, et al. 2021. Cell. 184(17):4464-4479.e19. PubMed
  4. Kieffer Y, et al. 2020. Cancer Discov. 1.340277778. PubMed
  5. Hu C, et al. 2021. Mol Med Rep. 1. PubMed
  6. Helmink BA, et al. 2020. Nature. 577:549. PubMed
  7. Zeng Y, et al. 2019. Cell Res. 29:881. PubMed
  8. Zeng Y et al. 2019. Immunity. 51(5):930-948 . PubMed
  9. Sade–Feldman M, et al. 2018. Cell. 175:998. PubMed
  10. He J, et al. 2021. Cell Res. 31:742. PubMed
  11. Martin GE, et al. 2018. Front Immunol. 1.019444444. PubMed
AB_2650977 (BioLegend Cat. No. 349115)
AB_2650978 (BioLegend Cat. No. 349116)

Antigen Details

Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer

Erythrocytes and erythroid precursors

Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.

Gene ID
2993 View all products for this Gene ID
View information about CD235a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 04/04/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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