FITC anti-human CD235a (Glycophorin A) Antibody

Pricing & Availability
HI264 (See other available formats)
Regulatory Status
VII 70312
Other Names
PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, Glycophorin A, MNS blood group, GYPA
Mouse IgG2a, κ
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Product Citations
Human red blood cells stained with HI264 FITC
  • HI264_FITC_061510
    Human red blood cells stained with HI264 FITC
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349103 25 µg 81€
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349104 100 µg 184€
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CD235a (Glycophorin A) is member of the glycophorin A family. It is a type I sialoglycoprotein with a molecular weight of 10 kD, present in the cell membrane as a homodimer. Glycophorin A is expressed by erythroid precursors and erythrocytes.  It carries the antigen determinants for the MNS blood groups and has been proposed to be an inhibitor of hemagglutination and hemolysis. Glycophorin A binds siglec 5, the erythrocyte binding antigen (EBA-175) of P. falciparum and some viruses, including influenza virus and hepatitis A virus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Mason D, et al. Eds. 2002. Leucocyte Typing VII:White Cell Differentiation Antigens. Oxford University Press. (FC)
Product Citations
  1. Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed
  2. Vinjamur DS, et al. 2021. Nat Genet. 53:719. PubMed
  3. Rao S, et al. 2021. Cell Stem Cell. 28(5):833-845.e5. PubMed
  4. Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed
  5. Wu Y, et al. 2019. Nat Med. 25:776. PubMed
  6. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  7. Lan X, et al. 2020. Molecular Cell. 81(2):239-254.e8. PubMed
  8. Heatlie J, et al. 2020. Biopreserv Biobank. 1.070833333. PubMed
  9. Ruiz JP, et al. 2019. Stem Cell Res. 41:101600. PubMed
  10. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  11. Zeng J, et al. 2020. Nat Med. 26:535. PubMed
  12. Martin RM et al. 2019. Cell Stem Cell. 24(5):821-828 . PubMed
  13. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  14. Lundin V, et al. 2020. Dev Cell. 446:52. PubMed
  15. Galle-Treger L, et al. 2019. Nat Commun. 10:713. PubMed
  16. Grajcarek J, et al. 2019. Nat Commun. 3.788888889. PubMed
  17. Weiss R, et al. 2021. Cells. 10:. PubMed
  18. Inglis H, et al. 2015. J Vis Exp. 97: 52484. PubMed
  19. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  20. Perik-Zavodskii R, et al. 2022. Genes (Basel). 13:. PubMed
AB_10612923 (BioLegend Cat. No. 349103)
AB_10613463 (BioLegend Cat. No. 349104)

Antigen Details

Member of the glycophorin A family; type I protein with a molecular weight of 10 kD; present in the membrane as homodimer

Erythrocytes and erythroid precursors

Possible inhibitor of hemagglutination and hemolysis; carries the antigen determinants for the MNS blood groups
Siglec 5, erythrocyte binding antigen (EBA-175) of P. falciparum, receptor for the influenza virus, hepatitis A virus
Cell Type
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Reid ME. 2009. Immunohematology 25:95.
2. Palacajornsuk P. 2006. Immunohematology 22:171.
3. Pasvol G. 2003. Trends Parasitol. 19:430.
4. Takakuwa Y. 2001. Curr. Opin. Hematol. 8:80.

Gene ID
2993 View all products for this Gene ID
View information about CD235a on

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Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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