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Anti-E. coli RNA Polymerase ImmunoAffinity Resin

Pricing & Availability
Clone
8RB13 (See other available formats)
Regulatory Status
RUO
Other Names
RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Previously
NeoClone Catalog# S0004
Isotype
Mouse IgG2b, κ
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Product Citations
publications
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673801 1 mL 259€
 
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Description

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant E. coli RNA polymerase β subunit.
Formulation
50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage & Handling
Store at 4°C until use. Use at room temperature.
Application

IP - Quality tested
Recommended Usage

Resin binding capacity is approximately 0.15 mg/mL. To elute, use TE, 0.7M ammonium sulfate, 40% propylene glycol.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.

Application References

(PubMed link indicates BioLegend citation)
  1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
  2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
  3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 11/11/2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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