Anti-E. coli RNA Polymerase ImmunoAffinity Resin

Pricing & Availability
8RB13 (See other available formats)
Regulatory Status
Other Names
RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
NeoClone Catalog# S0004
Mouse IgG2b, κ
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Product Citations
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673801 1 mL 259€
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RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Antibody Type
Host Species
Purified recombinant E. coli RNA polymerase β subunit.
50% anti- E. coli RNA Polymerase antibody conjugated resin is supplied in 1X PBS and 0.09% NaN3. The volume specified for each catalog number indicates the volume of resin included.
Storage & Handling
Store at 4°C until use. Use at room temperature.

IP - Quality tested

Recommended Usage

Resin binding capacity is approximately 0.15 mg/mL. To elute, use TE, 0.7M ammonium sulfate, 40% propylene glycol.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.

Application References

(PubMed link indicates BioLegend citation)
  1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
  2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
  3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)

Antigen Details

1252 amino acids with a predicted molecular weight of approximately 150 kD.


Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.

Gene ID
948488 View all products for this Gene ID
View information about RNA Polymerase beta on

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