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Purified anti-E. coli RNA Polymerase β Antibody

Pricing & Availability
Clone
8RB13 (See other available formats)
Regulatory Status
RUO
Other Names
RNAP subunit beta, rpoB, groN, nitB, rif, ron, stl, stv, tabD, RNA polymerase subunit beta, Transcriptase subunit beta
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
8RB13_PURE_RNAPolymerasebeta_102717_updated
Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone 8RB13). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder.
  • 8RB13_PURE_RNAPolymerasebeta_102717_updated
    Total lysates (15 µg protein) from E. coli BL21 cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 1:100000 diluted (0.005 µg/mL) purified anti-E. coli RNA Polymerase β antibody (clone 8RB13). Proteins were visualized using an HRP goat anti-mouse-IgG secondary antibody (clone Poly4053, Cat. No. 405306). Ponceau S staining (lower) was used as loading control. Lane M: Molecular weight ladder.
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663905 100 µg 259€
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Description

RNA polymerase (RNAP or RNApol) is an enzyme that produces primary transcript RNA. The β subunit is the second-largest subunit, and is encoded by the roB gene. RNA polymerase β is a subunit which, along wtih α (rpoC), limits core polymerase expression.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
E. coli, Bordetella, Chlamydiae, Myxococcus, Salmonella, Vibrio
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant E. coli RNA polymerase β subunit.
Formulation
0.22 µm filtered protein solution is in phosphate-buffered solution, pH 7.2.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. May be sub-aliquoted and stored at -20°C to avoid multiple freeze thaw cycles.
Application

WB - Quality tested
ELISA, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 - 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone 8RB13 is extremely useful for detecting or purifying core RNAP from a broad range of bacteria. The purified polymerase is highly active for in vitro transcription.

Additional reported applications (for the relevant formats) include: immunoprecipitation3. The epitope of clone 8RB13 has been mapped to the sequence PEEKLLRAIFGEKAS3.

Application References

(PubMed link indicates BioLegend citation)
  1. Bergendahl V, et al. 2003. Protein Expr. Purif. 31:155.
  2. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.
  3. Stalder, ES. et al. 2011. Protein Expr. Purif. 77(1):26-33. (Epitope, ELISA, IP, WB)
Product Citations
  1. d'Andrea FB, et al. 2022. Sci Adv. 8:eabn7943. PubMed
  2. Dupuy P, et al. 2020. Proc Natl Acad Sci U S A. 117:19517. PubMed
  3. Wipperman MF et al. 2018. Molecular cell. 72(1):152-161 . PubMed
  4. Olivencia B,et al. 2017. Sci Rep.. 10.1038/s41598-017-14410-z. PubMed
  5. Liang X, et al. 2021. PLoS Pathog. 17:e1010116. PubMed
  6. Hör J, et al. 2020. Nucleic Acids Res. 48:9301. PubMed
  7. Dupuy P, et al. 2022. Nat Commun. 13:4493. PubMed
  8. Liang X, et al. 2021. Appl Environ Microbiol. 87:e0043521. PubMed
RRID
AB_2566583 (BioLegend Cat. No. 663905)

Antigen Details

Structure
1252 amino acids with a predicted molecular weight of approximately 150 kD.
Distribution

Bacteria.

Function
Transcriptional regulator in bacteria.
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Thompson NE, et al. 1992. Biochemistry 31:7003.
2. Jerald Hurwitz, et al. 2005. J. Biol. Chem. 280:42477.
3. Hirata A, et al. 2008. Nature. 451:851.
4. Bergendahl IV, et al. 2003. Protein Expr. Purif. 31:155.
5. Burgess RR and Thompson NE. 2002. Curr. Opin. Biotechnol. 13:304.

Gene ID
948488 View all products for this Gene ID
UniProt
View information about RNA Polymerase beta on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 11/11/2020

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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