Alexa Fluor® 488 anti-human IL-31 Antibody

Pricing & Availability
Clone
1D10B31 (See other available formats)
Other Names
Interleukin-31
Isotype
Mouse IgG2b, κ
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Product Citations
publications
1D10B31_A488_IL31_Antibody_1_041516
Six hours PMA + Ionomycin-stimulated human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, fixed, permeabilized, and then stained with IL-31 (clone 1D10B31) Alexa Fluor® 488 (top) or mouse IgG2b, κ Alexa Fluor® 488 isotype control (bottom).
  • 1D10B31_A488_IL31_Antibody_1_041516
    Six hours PMA + Ionomycin-stimulated human peripheral blood lymphocytes (in the presence of monensin) were stained with CD3 FITC, fixed, permeabilized, and then stained with IL-31 (clone 1D10B31) Alexa Fluor® 488 (top) or mouse IgG2b, κ Alexa Fluor® 488 isotype control (bottom).
  • 1D10B31_A488_IL31_Antibody_2_041516
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
659607 25 tests 100€
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659608 100 tests 252€
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Description

IL-31, produced mainly by activated CD4+ T cells, is a newly discovered helical cytokine belonging to the gp130/IL6 cytokine family. IL-31 is closely related in structure to IL-6, IL-11, and IL-27. Binding of IL-31 to its receptor activates JAK/STAT, PI3K/AKT, and MAPK pathways. IL-31 acts on a broad range of immune and non-immune cells and thus possesses potential pleiotropic physiological functions, such as regulating hematopoiesis and immune response. Human IL-31 is also involved in inflammatory bowel disease, airway hypersensitivity, and dermatitis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full length human IL-31 recombinant protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
RRID
AB_2616660 (BioLegend Cat. No. 659607)
AB_2616661 (BioLegend Cat. No. 659608)

Antigen Details

Structure
161 aa with with a putative 23 aa signal peptide that is cleaved to generate a 138 aa mature protein.
Distribution
Secreted by activated CD4 T cells.
Function
IL-31 belongs to the IL-6 family of cytokines and plays a role in skin inflammation.
Ligand/Receptor
Binds to IL-31 receptor complex that consists of two receptor subunits, IL31RA and OSMR.
Cell Type
T cells, Eosinophils
Biology Area
Cell Biology, Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. McCandless EE, et al. 2014. Vet. Immunol. Immunopathol. 157:42.
2. Arai I, et al. 2013. Exp. Dermatol. 22:669.
3. Dai X, et al. 2013. PLoS One. 8:e67666.
4. Stott B, et al. 2013. J. Allergy Clin. Immunol. 132:446.
5. Hartmann K, et al. 2013. J. Allergy Clin. Immunol. 132:232.
6. Baron JM and Lüscher B. 2012. Acta. Derm. Venereol. 92:5.

Gene ID
386653 View all products for this Gene ID
UniProt
View information about IL-31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/15/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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