Purified anti-Ahr Antibody

Pricing & Availability
Clone
W16012A (See other available formats)
Regulatory Status
RUO
Other Names
Aryl hydrocarbon receptor, Ah receptor, AHR, Ah, Ahh, Ahre, bHLHe76
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W16012A_PURE_Ahr_Antibody_WB_031519_updated.png
Whole cell extracts (15 µg protein) from NIH3T3 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting Ahr (siAhr) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-AHR Antibody, clone W16012A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
  • W16012A_PURE_Ahr_Antibody_WB_031519_updated.png
    Whole cell extracts (15 µg protein) from NIH3T3 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting Ahr (siAhr) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of Purified anti-AHR Antibody, clone W16012A, overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular Weight marker.
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694502 100 µg 276 CHF
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Description

The Aryl hydrocarbon receptor (AHR) is a member of the basic helix–loop–helix–PER– ARNT–SIM (bHLH–PAS) subgroup of the bHLH superfamily of transcription factors and is the only member of this family known to be activated by ligands. Those ligands can be endogenous, naturally occurring, and/or anthropogenic. In vitro studies and gene expression profiles showed that the transcriptional changes by the activated Ahr are ligand specific and are highly cell specific. There is only one ligand binding pocket in the AHR (in the Per-ARNT-Sim-B domain), and the affinity of the best characterized high affinity ligand, TCDD (2,3,7,8-tetrachlorodibenzo-pdioxin), depends on only a few amino acids in that pocket. The Ahr can also be activated by numerous stress factors and substances that may not fit into the binding pocket, such as hyperoxia, oxidized low-density lipoproteins, hydrogen peroxide, ozone, and metals. Ahr regulates genes involved in a wide variety of biochemical pathways, including energy metabolism, lipid and cholesterol synthesis, xenobiotic metabolism and various transporters. The Ahr is well known to be involved in a variety of cellular processes, such as the cell cycle, epithelial barrier function, cell migration, and immune function.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial mouse Ahr (635-848 a.a.) expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB - Verified

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone W16012A does not cross-react with human (in-house tested).

Product Citations
  1. Gonder S, et al. 2021. Cancers (Basel). 13:. PubMed
  2. Amobi-McCloud A, et al. 2021. Front Immunol. 12:678999. PubMed
RRID
AB_2650731 (BioLegend Cat. No. 694502)

Antigen Details

Structure
848 amino acids with a predicted molecular weight of 95 kD.
Distribution

Cytoplasm and nucleus.

Function
Ligand-activated transcriptional activator. Binds to the XRE promoter region of genes it activates. Activates the expression of multiple phase I and II xenobiotic chemical metabolizing enzyme genes (such as the CYP1A1 gene). Mediates biochemical and toxic effects of halogenated aromatic hydrocarbons. Involved in cell-cycle regulation. Regulates the circadian clock by inhibiting the basal and circadian expression of the core circadian component PER1. Inhibits PER1 by repressing the CLOCK-ARNTL/BMAL1 heterodimer mediated transcriptional activation of PER1.
Interaction
In the nucleus, heterodimer of AHR and ARNT. Interacts with coactivators including SRC-1, RIP140 and NOCA7, and with the corepressor SMRT. Interacts with MYBBP1A, NEDD8, ARNTL/BMAL1.
Ligand/Receptor
Hydrophobic aromatic compound.
Biology Area
Cell Biology, Transcription Factors
Antigen References

1. Bersten DC, et al. 2013. Nat. Rev. Cancer 13:827.
2. Murray IA, et al. 2014. Nat. Rev. Cancer 14:801.
3. Reisz-Porszasz S, et al. 1994. Mol. Cell. Biol. 14(9):6075.
4. Esser C, Rannug A. 2015. Pharmacol. Rev. 67:259.
5. Sun YV, et al. 2004. Nucleic Acids Res. 32:4512.
6. Whelan F, et al. 2010. Mol. Pharmacol. 77:1037. 

Gene ID
11622 View all products for this Gene ID
UniProt
View information about Ahr on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All Ahr Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-Ahr W16012A WB,KO/KD-WB
PE anti-Ahr W16012A ICFC
Go To Top Version: 3    Revision Date: 10.29.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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