PE anti-mouse CD285 (TLR5) Antibody

Pricing & Availability
Clone
ACT5 (See other available formats)
Other Names
Toll-like receptor 5, TIL3, SLEB1, MELIOS
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
ACT5_PE_CD285_TLR5_Antibody_021221
Collagenase-digested C57BL/6 mouse splenocytes were stained with CD11c FITC and anti-mouse CD285 (TLR5) (clone ACT5) PE (left) or mouse IgG1, κ PE isotype control (right).
  • ACT5_PE_CD285_TLR5_Antibody_021221
    Collagenase-digested C57BL/6 mouse splenocytes were stained with CD11c FITC and anti-mouse CD285 (TLR5) (clone ACT5) PE (left) or mouse IgG1, κ PE isotype control (right).
See PE spectral data
Cat # Size Price Quantity Avail. Save
148107 25 µg 145 CHF
 
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148108 100 µg 305 CHF
 
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Description

CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
TLR5-transfected Ba/F3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Shibata T, et al. Int Immunol. 24:613. (IP, FC)
  2. Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)
RRID
AB_2890696 (BioLegend Cat. No. 148107)
AB_2890696 (BioLegend Cat. No. 148108)

Antigen Details

Structure
Type 1 transmembrane protein, 98 kD
Distribution

Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium

Interaction
Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
Ligand/Receptor
Flagellin
Cell Type
Dendritic cells, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587. 
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857. 
3. Carapau D, et al. 2013. Infect. Immun. 81:4350. 
4. Kinnebrew M, et al. 2012. Immunity. 36:276. 
5. Sebastiani G, et al. 2000. Genomics. 64:230.

Gene ID
53791 View all products for this Gene ID
UniProt
View information about CD285 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 02.05.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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