- ACT5 (See other available formats)
- Other Names
- Toll-like receptor 5, TIL3, SLEB1, MELIOS
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
|Cat #||Size||Price||Quantity Avail.||Save|
|148107||25 µg||145 CHF|
|148108||100 µg||305 CHF|
CD285, also known as TLR5, is a 98 kD pattern recognition receptor that binds to bacterial flagellin. It is expressed on dendritic cells, classical monocytes and mucosal epithelial cells and is involved in activation of TLR5 induced NF-κB mediated activation of inflammatory-related target genes. Mutations in the TLR5 gene have been linked with susceptibility to systemic lupus erythematosus and Legionnaire’s disease.Product Details
- Antibody Type
- Host Species
- TLR5-transfected Ba/F3 cells
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications for the relevant formats include: immunoprecipitation of TLR5 from cell lysates1,2.
(PubMed link indicates BioLegend citation)
- Shibata T, et al. Int Immunol. 24:613. (IP, FC)
- Huh JW, et al. 2014. Proc. Natl. Acad. Sci. USA. 111:7072. (IP)
AB_2890696 (BioLegend Cat. No. 148107)
AB_2890696 (BioLegend Cat. No. 148108)
- Type 1 transmembrane protein, 98 kD
Neutrophils, CD11b, Ly6C, classical monocytes, dendritic cells, mucosal epithelium
- Interacts with MyD88 and TRAF6 leading to NF-κB activation and production of cytokines.
- Cell Type
- Dendritic cells, Monocytes, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
1. López-Yglesias AH, et al. 2014. J. Immunol. 192:1587.
2. Burdelya L, et al. 2013. Proc. Natl. Acad. Sci. USA. 110:E1857.
3. Carapau D, et al. 2013. Infect. Immun. 81:4350.
4. Kinnebrew M, et al. 2012. Immunity. 36:276.
5. Sebastiani G, et al. 2000. Genomics. 64:230.
- Gene ID
- 53791 View all products for this Gene ID
- View information about CD285 on UniProt.org
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
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