Ultra-LEAF™ Purified anti-human CD48 Antibody

Pricing & Availability
Clone
W20019A (See other available formats)
Regulatory Status
RUO
Other Names
CD48 antigen, B-lymphocyte activation marker BLAST-1, Blast-1, SLAMF2, HuLy-m3, BCM1 surface antigen, Leukocyte antigen MEM-102, SLAM family member 2, Signaling lymphocytic activation molecule 2, TCT.1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W20019A_ULEAF_CD48_Antibody_011322
Biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc Chimera (black circles) binds to immobilized recombinant human CD48 (SLAMF2)-Fc chimera (Cat. No. 788706). Ultra-LEAF™ purified anti-human CD48 (clone W20019A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 7 µg/mL biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc chimera to 5 µg/mL immobilized recombinant human CD48 (SLAMF2)-Fc Chimera. ND50 range: 0.04 - 0.2 µg/mL
  • W20019A_ULEAF_CD48_Antibody_011322
    Biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc Chimera (black circles) binds to immobilized recombinant human CD48 (SLAMF2)-Fc chimera (Cat. No. 788706). Ultra-LEAF™ purified anti-human CD48 (clone W20019A) (purple squares) inhibits the binding in a dose-dependent manner, whereas the GoInVivo™ purified rat IgG2a, κ isotype control (Cat. No. 400562) (gray triangles) does not have the effect. This antibody blocks the binding of 7 µg/mL biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc chimera to 5 µg/mL immobilized recombinant human CD48 (SLAMF2)-Fc Chimera. ND50 range: 0.04 - 0.2 µg/mL
Cat # Size Price Quantity Check Availability Save
947103 100 µg 215 CHF
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947104 1 mg 864 CHF
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Description

CD48, also known as SLAMF2, is one of the members of the CD2 subfamily. CD48 is a GPI-anchored membrane protein containing two Ig-like C2 type domains and is widely expressed on both resting and activated hematopoietic cells with the exception of granulocytes, platelets, and erythrocytes. CD48 surface expression is elevated upon viral infection. Engagement of SLAMF4 (CD244) by a natural ligand, CD48, leads to cell-mediated cytotoxicity and IFN-γ secretion by NK cells via the adaptor protein, SAP. While CD48-expressing tumors were efficiently eliminated by NK cells in SLAMF4-deficient mice, indicating that the CD48/SLAMF4 interaction plays an inhibitory role on NK cells in a context-dependent manner. In mice lacking expression of self-MHC-binding inhibitory receptors, ligation of CD48 and SLAMF4 inhibits NK cell activation, indicating that CD48/SLAMF4 contributes to NK-cell self-tolerance in a MHC-I-independent manner. Differential expression of CD48 in distinct subsets of human dendritic cells affects NK cell activation. In addition, CD48 interacts with SLAMF4 and functions as a costimulatory molecule, playing a critical role in cell proliferation and MHC-restricted cytotoxicity in CD8+ T cells. CD48 on the surface of marginal zone B cells activates NK cells and stimulates Th2 cytokine IL-13 production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human CD48 (SLAMF2)-Fc chimera
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Block - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by blocking the binding of 7 µg/mL biotinylated recombinant human SLAMF4 (CD244/2B4)-Fc chimera to 5 µg/mL immobilized recombinant human CD48 (SLAMF2)-Fc chimera (Cat. No. 788706). ND50 range: 0.04 - 0.2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application. 

RRID
AB_2910509 (BioLegend Cat. No. 947103)
AB_2910509 (BioLegend Cat. No. 947104)

Antigen Details

Structure
Disulfide-linked homodimer
Distribution

Widely expressed on hematopoietic cells except granulocytes, platelets, and erythrocytes. Increased on T and B cells after activation

Function
Contributes to T cell antigen recognition, plays an inhibitory and activating role on NK cells
Interaction
NK cells, subsets of T cells; Lck, Fyn interact with cytoplasmic domain
Ligand/Receptor
SLAMF4 (CD244)
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Veillette A. 2004. J Exp Med. 199:1175-8.
  2. Fisher RC and Thorley-Lawson DA. 1991. Mol Cell Biol. 11:1614-23.
  3. Yokoyama S, et al. 1991. J Immunol. 146:2192-200.
  4. Lee KM, et al. 2004. J Exp Med. 199:1245-54.
  5. Assarsson E, et al. 2005. J Immunol. 175:2045-9.
  6. Korinek V, et al. 1991. Immunogenetics. 33:108-12. 
  7. Knapp W, et al. 1989. Immunol Today. 10:253-8. 
  8. Schlossman S, et al. 1995. Oxford University Press
  9. McNerney ME, et al. 2005. Blood. 106:1337-40. 
  10. Gao N, et al. 2006. J Immunol. 176:2758-64.
  11. Morandi B, et al. 2005. J Immunol. 175:3690-7.
  12. Messmer B, et al. 2006. J Immunol. 176:4646-50.
  13. Lee KM, et al. 2003. J Immunol. 170:4881-5.
  14. Kato K, et al. 1992. J Exp Med. 176:1241-9.
  15. Lee KM, et al. 2006. Blood. 107:3181-8. 
Gene ID
962 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-human CD48

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