Purified anti-STAT1 Antibody

Pricing & Availability
Clone
10C4B40 (See other available formats)
Other Names
Signal transducer and activator of transcription 1 (STAT1), Transcription factor ISGF-3 components p91/p84
Isotype
Mouse IgG1, κ
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Product Citations
publications
10C4B40_PURE_STAT1_Antibody_WB_1_120417
Total lysates (15 µg protein) from U87-MG, HeLa, and Daudi cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-STAT1 antibody, clone 10C4B40. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
  • 10C4B40_PURE_STAT1_Antibody_WB_1_120417
    Total lysates (15 µg protein) from U87-MG, HeLa, and Daudi cells were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 (1 µg/ml) purified anti-STAT1 antibody, clone 10C4B40. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin antibody was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # Size Price Quantity Avail. Save
661001 25 µg 75 CHF
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661002 100 µg 225 CHF
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Description

STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated by receptor-associated kinases, translocates to the nucleus, and functions as a transcription factor. Two isoforms of STAT1, with apparent molecular weights of 88 and 91 kD, exist as a result of alternative RNA processing. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 µg per ml (1:500). It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).

RRID
AB_2563663 (BioLegend Cat. No. 661001)
AB_2563664 (BioLegend Cat. No. 661002)

Antigen Details

Structure
750 amino acids, predicted molecular weight of 87 kD; contains a SH2 domain responsible for homodimerization or heterodimerization.
Distribution

Cytoplasm; phosphorylated form translocates to the nucleus.

Function
Phosphorylated in response to cytokine signaling by receptor-associated kinases; translocates to the nucleus to act as a transcription factor. Mediates responses to type I (IFN-α/β) and II interferon (IFN-γ), EGF, PDGF, and IL-6.
Interaction
Forms a homodimer or heterodimers with other family members. Interacts with FAK, MCM3, MCM5, TRADD, BRCA1, KIT, IL-27R, IL-2Rβ, IL-2Rγ, IFNαβR, and c-Src.
Cell Type
Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction, Stem Cells, Transcription Factors
Antigen References

1. Durbin JE, et al. 1996. Cell 8:443.
2. Darnell JE Jr, et al. 1994. Science 264:1415.
3. Chen X, et al. 1998. Cell 93:827.
4. Ramana CV, et al. 2000. Oncogene 19:2619.

Gene ID
6772 View all products for this Gene ID
UniProt
View information about STAT1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 12.04.2017

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