Purified anti-mouse CD123 Antibody

Pricing & Availability
5B11 (See other available formats)
Regulatory Status
Other Names
IL-3 Receptor α chain, IL-3Rα
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse bone marrow cells stained with 5B11 PE and CD11b FITC
  • 5B11_080505
    C57BL/6 mouse bone marrow cells stained with 5B11 PE and CD11b FITC
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106002 500 µg 229 CHF
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CD123 is a 70 kD α chain subunit of the IL-3 receptor (IL-3R α). It is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL-3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either the common β chain (CDw131) or the IL-3R β chain (AIC2A) to form high-affinity heterodimeric IL-3 receptors. CDw131 can complex with the α subunits of the mouse IL-3R, IL-5R and GM-CSFR to form high-affinity receptors, while the IL-3 R β subunit is specific for IL-3 but binds with low affinity. IL-3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. The 5B11 antibody does not block binding of IL-3 to the high affinity IL-3 receptor.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
CTLL cells transfected with the mouse IL-3Rα chain
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 106006) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 106004) or biotinylated anti-rat IgG second step (Cat. No. 405402), followed by SAv-PE (Cat. No. 405204)). To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining.

Application References

(PubMed link indicates BioLegend citation)
  1. Ichihara, et al. 1995. EMBO J. 14:939. (FC)
  2. Mueller DL, et al. 1994. J. Immunol. 153:3014. (FC)
  3. Cervantes-Barragan L, et al. 2007. Blood 109:1131. (FC)
Product Citations
  1. Joseph R, et al. 2021. Br J Cancer. 125:176. PubMed
  2. Matsuba S, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01538. PubMed
  3. Singh K, et al. 2018. J Immunol. 200:595. PubMed
AB_2124405 (BioLegend Cat. No. 106002)

Antigen Details

Ig superfamily, associates with CDw131 (common β chain) or IL-3 receptor β chain, 70 kD

Hematopoietic progenitors, basophils, mast cells, megakaryocytes

Proliferation, differentiation of hematopoietic cells
Cell Type
Basophils, Dendritic cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Miyajima A, et al. 1993. Blood 82:1960.
3. Itoh N, et al. 1990. Science 247:324.

Gene ID
16188 View all products for this Gene ID
View information about CD123 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse CD123

  • PE anti-mouse CD123

  • Purified anti-mouse CD123


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