- HECA-452 (See other available formats)
- Regulatory Status
- V S075
- Other Names
- Cutaneous Lymphocyte-associated Antigen (CLA)
- Rat IgM, κ
- Ave. Rating
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- Product Citations
|Cat #||Size||Price||Quantity Check Availability||Save|
|321302||100 µg||100,00 CHF|
Cutaneous lymphocyte antigen (CLA) is a 140 kD homodimer protein recognized by a unique mAb, HECA-452. It is expressed on T cells in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells as well as on monocytes, granulocytes, and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on the majority of peripheral blood leukocytes. CLA is a ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering, and rolling.Product Details
- Human, Mouse
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
FC - Quality tested
IHC-P - Verified
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per million cells in 100 µl volume. For immunohistochemistry formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The HECA-452 antibody cross-reacts with mouse skin homing lymphocytes4. Treatment of activated HUVEC cells with HECA-452 antibody inhibits lymphocyte adhesion. Additional reported applications (for the relevant formats) include: blocking of lymphocyte binding to E-selectin3, and immunohistochemistry1,2 of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections.
(PubMed link indicates BioLegend citation)
- Duijvestijn AM, et al. 1988. Am. J. Pathol. 130:147. (IHC)
- Picker LJ, et al. 1991. Nature 349:796. (IHC)
- Berg EL, et al. 1991. J. Exp. Med. 174:1461.
- Borges E, et al. 1997. J. Biol. Chem. 272:28786.
- Ren YL, et al. 2012. Am J Clin Pathol. 138:435. PubMed
- Product Citations
AB_492894 (BioLegend Cat. No. 321302)
- 140 kD, carbohydrate epitope of PSGL-1
T cells in skin, subsets of peripheral blood T cells, NK cells, B cells and dendritic cells, monocytes, granulocytes
- Memory cells tethering and rolling
- E-selectin, P-selectin, L-selectin
- Cell Type
- B cells, Dendritic cells, Granulocytes, Monocytes, NK cells, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Picker LJ, et al. 1990. Am. J. Pathol. 136:1053.
2. Berg EL, et al. 1991. J. Exp. Med. 174:1461.
3. Fuhlbrigge RC, et al. 1997. Nature 389:978.
4. Tu L, et al. 1999. J. Exp. Med. 189:241.
5. Yoshino T, et al. 1999. Cell. Immunol. 197:39.
6. Chang SE, et al. 2003. Acta Derm-Venereol. 83:162.
7. Schakel K, et al. 2002. Immunity 17:289.
8. Fuhlbrigge RC, et al. 2002. J. Immunol. 168:5645.
- Gene ID
- 6404 View all products for this Gene ID
- View information about CLA on UniProt.org
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