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Purified anti-Dicer Antibody (Previously Covance catalog# MMS-5130)

Pricing & Availability
Clone
N167/7 (See other available formats)
Regulatory Status
RUO
Other Names
Endoribonuclease Dicer, Dicer1, Dcr-1 homolog, double-strand-specific ribonuclease mDCR-1, Helicase with RNase motif, HERNA, Helicase MOI, KIAA0928
Previously
Covance Catalog# MMS-5130
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
N167slash7_Purified_Dicer_Antibody_1_040319_updated.png
Western blot of purified anti-Dicer antibody (clone N167/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of MCF-7 cell lysate; Lane 3: 20 µg of mouse 3T3 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N167slash7_Purified_Dicer_Antibody_1_040319_updated.png
    Western blot of purified anti-Dicer antibody (clone N167/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of MCF-7 cell lysate; Lane 3: 20 µg of mouse 3T3 cell lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Check Availability Save
820201 100 µL 165 CHF
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Description

Dicer, also known as double-strand-specific ribonuclease (DCR-1) and helicase with RNase motif (HERNA), processes double-stranded RNA and pre-microRNA (miRNA) to generate siRNA and miRNA, respectively. These short RNA sequences mediate RNA silencing through RISC (RNA-induced silencing complex).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1638-1899 of mouse Endoribonuclease Dicer.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1.0 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Vishlaghi N, et al. 2020. Front Cell Dev Biol. 8:338. PubMed
RRID
AB_2564838 (BioLegend Cat. No. 820201)

Antigen Details

Structure
Expected MW: 215 kD
Biology Area
Cell Biology, Neuroscience
Gene ID
192119 View all products for this Gene ID
UniProt
View information about Dicer on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 08.15.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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