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PE/Cyanine7 anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_PECy7_110210
C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cyanine7.
  • PK136_PECy7_110210
    C57BL/6 mouse splenocytes were stained with DX5 APC and NK1.1 (clone PK136) PE/Cyanine7.
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
108713 25 µg 87 CHF
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108714 100 µg 198 CHF
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Additional Product Notes
BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  2. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  3. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  4. Tran NT, et al. 2019. Cell Rep. 28:3510. PubMed
  5. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  6. Wu N, et al. 2020. Cell Rep. 30:1129. PubMed
  7. Zhou J, et al. 2019. Immunity. 50:403. PubMed
  8. Du H et al. 2019. Cancer cell. 35(2):221-237 . PubMed
  9. Piepke M, et al. 2021. J Neuroinflammation. 18:265. PubMed
  10. Yu K, et al. 2021. Cell Reports. 35(12):109273. PubMed
  11. Fu B et al. 2017. Immunity. 47(6):1100-1113 . PubMed
  12. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  13. Earnest JT, et al. 2021. Cell Reports. 35(1):108962. PubMed
  14. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  15. Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
  16. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  17. Dai B, et al. 2022. Theranostics. 12:7603. PubMed
  18. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  19. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  20. Yang X, et al. 2019. Oncotarget. 10:4180. PubMed
  21. Cong M, et al. 2021. Methods Mol Biol. 2388:59. PubMed
  22. Robbins G, et al. 2012. J Biol Chem. 287:24294. PubMed
  23. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  24. Qu J, et al. 2018. J Immunol Res. 2018:7519856. PubMed
  25. Chun E, et al. 2020. Immunity. 51(5):871-884.e6.. PubMed
  26. Cong J et al. 2018. Cell metabolism. 28(2):243-255 . PubMed
  27. Hutter K, et al. 2020. FEBS J. . PubMed
  28. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  29. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  30. McFarland AP, et al. 2021. Immunity. 54(6):1320-1337.e4. PubMed
  31. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  32. Liu W, et al. 2019. Epigenetics. 1.192361111. PubMed
  33. Wu B, et al. 2020. Int J Biol Sci. 16:1526. PubMed
  34. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  35. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  36. Ma W, et al. 2017. Cell Death & Disease. 10.1038/cddis.2017.47. PubMed
  37. Zhang X, et al. 2021. Mol Cancer Res. 19:1076. PubMed
  38. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  39. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  40. Itoga M, et al. 2015. PLoS One. 10: e0123210. PubMed
  41. Bitschar K, et al. 2019. Nat Commun. 10:2730. PubMed
  42. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  43. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  44. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  45. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  46. Hayashida E, et al. 2019. J Neuroinflammation. 0.789583333. PubMed
  47. Verma M, et al. 2021. J Exp Med. 218:. PubMed
  48. Boulch M, et al. 2021. Sci Immunol. 6:. PubMed
  49. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  50. Hsia HC, et al. 2017. J Leukoc Biol. 101:1053. PubMed
  51. Leruste A, et al. 2020. Cancer Cell. 36(6):597-612.e8.. PubMed
RRID
AB_389363 (BioLegend Cat. No. 108713)
AB_389364 (BioLegend Cat. No. 108714)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12.08.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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