PE anti-mouse CD68 Antibody

Pricing & Availability
Clone
FA-11 (See other available formats)
Regulatory Status
RUO
Other Names
Macrosialin
Isotype
Rat IgG2a
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Product Citations
publications
FA-11_PE_062310
Thioglycolate-elicited BALB/c peritoneal macrophages intracelluarly stained with FA-11 PE
  • FA-11_PE_062310
    Thioglycolate-elicited BALB/c peritoneal macrophages intracelluarly stained with FA-11 PE
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Cat # Size Price Quantity Check Availability Save
137013 25 µg 141 CHF
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137014 100 µg 335 CHF
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Description

Mouse CD68, also known as macrosialin, is an 85-115 kD member of the lysosomal-associated membrane protein (LAMP) family. It is a heavily glycosylated and predominantly intracellular protein, mainly in late endosomes. Macrosialin is the murine homolog to the human macrophage glycoprotein CD68. It is expressed on tissue macrophages, Langerhans cells and at low levels on dendritic cells. Lamp proteins may have functions relating to cell-cell interaction or cell-ligand interaction. The biological function of CD68 is not completely understood.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2, immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  10. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Zhao C, et al. 2013. Int J Pharm. 450:11. PubMed
  2. Miho Sato‐Hashimoto et al. 2019. eLife. 8 pii: e42025. PubMed
  3. Deng R, et al. 2022. Adv Sci (Weinh). 9:e2103343. PubMed
  4. Bhat N, et al. 2022. J Clin Invest. 132:. PubMed
  5. Zhang Y, et al. 2013. J Immunol. 900:4725. PubMed
  6. Wei SC, et al. 2020. Cancer Discov. . PubMed
  7. Guan Z, et al. 2020. Front Cell Neurosci. 0.763888889. PubMed
  8. Li Y, et al. 2021. Brain Behav Immun. 91:267. PubMed
  9. Cougnoux A, et al. 2018. Hum Mol Genet. 27:2076. PubMed
  10. Cummings H, et al. 2012. Proc Natl Acad Sci U S A. 109:1251. PubMed
  11. Lv LL, et al. 2018. J Am Soc Nephrol. 29:919. PubMed
  12. Litvinchuk A, et al. 2021. Ann Neurol. 89:952. PubMed
  13. Litvinchuk A et al. 2018. Neuron. 100(6):1337-1353 . PubMed
  14. Grisanti LA, et al. 2019. JCI Insight. 5. PubMed
  15. Osinski V, et al. 2020. Theranostics. 10:585. PubMed
  16. Li C, et al. 2020. Cancer Immunol Res. 1.030555556. PubMed
  17. O'Boyle C, et al. 2020. Int J Stroke. 0.746527778. PubMed
  18. Fusciello M, et al. 2019. Nat Commun. 4.407638889. PubMed
RRID
AB_10613469 (BioLegend Cat. No. 137013)
AB_10612937 (BioLegend Cat. No. 137014)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 04.26.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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